CP对大鼠全血AChE活性影响.pdfVIP

第十一届中南地区实验动物科技交流会论文集 CP 对大鼠全血 AChE 活性的影响 姚茂忠 符 健* （海南医学院海南省药物安全性评价研究中心海南海口 571101 ） 【摘要】目的探讨氯氰菊酯（CP ）慢性染毒后大鼠全血乙酰胆碱酯酶（AChE ）活性的 变化规律。 方法 120 只 80-100g 雄性 SD 大鼠随机分为 4 组， 即对照组（花生油）、低剂 量组（40 mg/kg CP ）、中剂量组（80 mg/kg CP）和高剂量组（160 mg/kg CP），每组30 只。 灌胃给药 28 次，每日 1 次，共 28 日，每日观察动物反应症状。给药前、给药后7、14、21 、 28 和给药结束后 7 日各组断尾取血，测定全血AChE 活性。 结果 CP 染毒后大鼠表现出神 经受损症状，同时大鼠全血 AChE 活性显著降低（P ﹤0.05 ），且呈现剂量依赖性。 结论 CP 慢性染毒后大鼠全血 AChE 活性显著降低，呈现剂量和时间依赖性，表明其具备成为神经损 伤标志物的潜力。 【关键词】CP ； 大鼠； AChE ； 活性 Effect of CP on AChE activity of whole blood in Rats 1 2 3 YAO Mao-zhong , LI Lin-fang , CHEN Zhan-juan ，FU Jian (1 Research Center for Drug Safety Evaluation of Hainan Province, Hainan Medical College, Haikou 571101, China;2 Simcere Pharmaceutical Co.L.T.D. Haikou 570216, China;3 College of Chemistry and Chemical engineering, Hainan Normal University, Haikou 571158, China) 【Abstract 】Objective To investigate variation of Acetylcholinesterase (AChE) activity of whole blood after rat’s chronic exposure to cypermethrin (CP). Methods 120 male SD rats (80-100g) were equally divided into 4 groups, including control group (peanut oil), low dosage group (40 mg cp /kg body), middle dosage group (80 mg cp /kg body) and high dosage group(160 2 mg cp /kg body). Intragastric administration at 1 time a day were planed, the treatment lasted 28 days. General symptoms of rat were observed everyday. Blood was collected by tail abscise at 0, 7,14,21,28 day after treatment and 7 days after last treatment, respectively. Activity of whole blood AChE were measured. Results Rats showed symptoms of nerve damage, and activity of whole blood AChE was decreased signific