纳豆激酶分离纯化技术的研究进展.pdfVIP

第 卷第 期 现代化工 Feb . 　 年 月 Modern Chemical Industry ·23 · 纳豆激酶分离纯化技术的研究进展 阳承利 　邢建民　刘俊果 　刘先桥 　刘会洲 ( 中国科学院过程工程研究所分离科学与工程实验室 ,生化工程国家重点实验室 ,北京 100080) 摘要 :对纳豆激酶的生产和分离纯化技术进行了综述 ,重点讨论了柱层析 、发酵与泡载分离耦合和膨胀床吸附等分离方法 , 提出了将反胶团和磁性高分子微球等新型分离技术应用于纳豆激酶的分离以提高其纯度和产率 ,指出了纳豆激酶有望开发成 一种新一代理想的溶栓剂 ,具有广阔的应用前景 。 关键词 :纳豆激酶 ;分离纯化 ;反胶团;磁性高分子微球 ( ) 中图分类号 :Q55 　 文献标识码 :A 　 文章编号 :0253 - 4320 2004 02 - 0023 - 03 Progress in separation and purif ication technology of nattokinase YAN G Chengli , XIN G J ianmin , L I U J unguo , L I U X ianqiao , L I U Huiz hou (Laboratory of Separation Science and Engineering , State Key Laboratory of Biochemical Engineering , Institute of Process Engineering , Chinese Academy of Sciences , Beijing 100080 , China) Abstract : The research on production and separation and purification of nattokinase were reviewed. Column chromatogra phy ,coupling between fermentation and foam separation ,and expand bed adsorption were discussed. New separation technologies such as reversed micelles and magnetic polymer microspheres for application in nattokinase were put forward in order to improve its purity and yield. It was pointed out that nattokinase would be developed a new and ideal thrombolytic agents with extensive application prospects. Key words : nattokinase ; separation and purification ; reversed micelle ; magnetic polymer microsphere 　　1987 年 日本学者 H Sumi 等从 日本传统的大豆 纳豆中分离提取的[4 ] 。