毛脉酸模不同器官中几种生物活性物质的含量及生长季节对其的影响.pdfVIP

植物学通报 2005, 22 (5): 572~578 Chinese Bulletin of Botany 实验简报 毛脉酸模不同器官中几种生物活性物质的 含量及生长季节对其的影响① ② 王振月 崔红花 康毅华 李瑞明 王宗权 (黑龙江中医药大学药学院 哈尔滨 150040) 摘要 为确定毛脉酸模(Rumex gmelini )最佳药用部位和最佳采收期, 从而为新药源开发提供科学依 据, 我们采用HPLC法和梯度洗脱, 研究了一年生和二年生毛脉酸模不同生长发育期、不同器官、组织 中7种生物活性成分( 白藜芦醇、白藜芦醇苷、大黄酚苷、酸模素、大黄素、大黄酚和大黄素甲醚) 的动 态分布规律。结果表明毛脉酸模根部含有生物活性成分的种类及含量均多于其他部位, 为最佳药用部 位。一年生毛脉酸模根部不含有酸模素。二年生毛脉酸模根部含有全部7种生物活性成分, 且在8~9月 份含量最高, 为最佳采收期。 关键词 毛脉酸模, 高效液相色谱, 蒽醌, 白藜芦醇 Content of Active Components of Organs of Rumex gmelini and the Influence by Growing Time WANG Zhen-Yue② CUI Hong-Hua KANG Yi-Hua LI Rui-Ming WANG Zong-Quan (College of Pharmacy , Heilongjiang University of Traditional Chinese Medicine, Harbin 150040) Abstract To confirm the most appropriate pharmaceutical source and harvest time and provide a scientific basis for exploiting Rumex gmelini, we used HPLC to examine the dynamic distribu- tion of 7 components (resveratrol, polydatin, chrysophanol, chrysophaein, emodin, physcion and musizin) in organs, tissues and development stages of 1- or 2-year-old Rumex gmelini . The kind and content of active components varied in roots as compared with other plant parts, with roots being the most appropriate pharmaceutical source. Musizin was not found in 1-year-old plants. All 7 active components were found in roots of 2-year-old plants, and the content was higher during August and September, so this period is the most appropriate harvest time for R. gmelini . Key words Rumex gmelini, HPLC, Anthroquinones, Resveratrol 毛脉酸模(Rumex gmelini)为蓼科酸模属多 吉林长白山地区的重要药用资源植物。酸模 年生宿根草本植物, 是黑龙江省、吉林省、 属的利用和研究领域较广(Midwo et al.,1985; ①国家自然科学基金和黑龙江省自然科学基金(B9811) 资助。 ②通讯作者。Author for correspondence. E-mail: wangzhen_yue@163.com 收稿日期: 2004-08-30 接受日期: 2005-02-21 责任编辑: 于昕 2005 王振月等: 毛脉酸模不同器官中几种生物活性物质的含量及生长季节对其的影响 573 Humphreys e