TTC染色识别家猪急性心肌梗死模型中存活心肌的研究.pdfVIP

# 334 # 2011425 4 Journal of Chinese Practical Diagnosis and Therapy # # TTC * 张 雷,木胡牙提, 林 涛,马依彤,杨毅宁, 仇 萍, 刘 芬, 张燕 一 (, 830054) [] :应用经皮腔内冠状动脉成 术球囊堵闭猪冠状动脉建立急性心肌梗死动物模型,并通过T TC 染色 识别急性心肌梗死动物模型中存活心肌和梗死心肌: 普通家猪 13 只,麻醉气管插管后行冠状动脉造影术, 置入经 皮腔内冠状动脉成 术球囊至冠状动脉左前降支远端,堵闭血流60~ 90 min,建立家猪心肌梗死模型处死试验动物取 出心脏,将心肌组织切成薄片后采用 TTC 染色法识别存活心肌: 家猪均完成冠状动脉左前降支远端的封堵, 2 只 家猪分别在堵闭45 min,65 min 时因心室颤动死亡,存活的11 只家猪均成功建立急性心肌梗死模型, TTC 染色显示正 常心肌88 个节段, 梗死心肌49 个节段, 存活心肌39 个节段: 运用经皮腔内冠状动脉成 术球囊封堵冠状动脉可 成功建立猪急性心肌梗死模型,通过 TTC 染色能识别出心肌梗死模型中的梗死心肌与存活心肌 [] 急性心肌梗死; 动物模型; 冠状动脉堵闭法;球囊; 存活心肌; TTC 染色 :R541.4 :A : 1674-3474( 2011)04-0334-03 TTC dyeing detecting viable myocardium in pig models with acute myocardial inf arction ZHAN Lei, MU Huyati, LIN T ao, MA Yi-tong, YAN Yi-ning, QIU Ping, LIU Fen, ZHAN Yan-Yi Cardiac Center, the First Affiliated Hospital to Xinjiang Medical University, Urumqi 830054, China [ Abstract] Objective T o identify viable myocardium and infarct myocardium with TT C dyeing technique by establishing pig models with acute myocardial infarction by using percutaneous transluminal coronary angioplasty sacculus. Methods T hirteen pigs received coronary angiography by inserting percutaneous transluminal coronary angioplasty sacculus to the end of the left anterior descending coronary artery after anesthesia tracheal intubation. T hen the blood flew was blocked for 60 to 90 minutes, and thepig models with acutemyocardial infarction were established. T hepigs were sacrificed and the hearts were taken out, then the heart tissue was cut to slices and recognized the viable myocardium with TTC dyeing