种群微卫星DNA分析中样本量对各种遗传多样性度量指标的影响_闫路娜.pdfVIP

　50(2):279-290, 2004 Acta Zoologica Sinica 　　　　　 　 DNA ＊ 闫路娜　张德兴＊＊ , , 　100080 　　, , :、 , ;; , 30-50 DNA 。 , 。 , , [ 50 (2):279-290, 2004] 。 　　　　　　 DNA 　　 Effects of sample size on various genetic diversity measures in population genetic study ＊ with microsatellite DNA markers ＊＊ YAN Lu-Na, ZHANG De-Xin State Key Laboratory of Inte rated Mana ement of Pest Insects and Rodents, Institute of Zoolo y, Chinese Academy of Sciences, Beijin 100080, China Abstract　Sample collection and samplin strate y is of primary importance in population enetic studies, and can reatly influence the analysis and interpretation of the data obtained.Microsatellite DNA sequences are the most revealin DNA markers available so far for inferrin population structure and dynamics, and have been widely employed in enetic studies of populations.Nevertheless, few studies have specifically examined the effects of sample size and other samplin factors on various enetic diversity measures in population enetic work usin these markers.When such issues were discussed in the literature, it was of ten very brief or not based on experimental data.Here, w e examined these issues more closely us- in empirical enotype data from an analy sis of 26 populations of the mi ratory locusts Locusta migratoria (1 381 individ- uals)at 8 microsatellite loci.The follow in enetic diversity measures w ere studied:number of alleles per locus (NA ), mean number of allele over loci (MNA), observed heterozy osity (Ho)and expected heterozy osity (He).We also tried to infer the theoretic minimum sample size needed for population enetic studies with microsatellite loci.Our main results and conclusions are as follows. (1)NA and MNA, i.e.the estimated allelic richness of populations, are si nificantl