流式细胞术测定血小板活化在先兆子痈孕妇中的临床应用.pdfVIP

·68 · ChineseJournalofThrombosisandHemostasis2005Vol11No2 流式细胞术测定血小板活化在先兆子痈孕妇中的临床应用 易正山.孟凡义，冯 茹，林 榕，宋兰林，周淑芸 (南方医科大学南方医院血液科，广州 510510) 摘 要: 目的 运用流式细胞术测定血小板的活化状态，探讨先兆子痛孕妇体内静息态与活化态血小板CD41a, CD62p,CD63等血小板膜糖蛋白表达量的变化及其临床意义。方法 用免洗的全血免疫直标法，将血小板CD41a,CD 62p,CD63荧光单抗分别标记25例正常人与20例正常孕妇及22例先兆子痈孕妇静息态及ADP活化后血小板，在流式 细胞仪上测定其表达量。结果 正常孕妇组与正常对照组比较，静息态间及活化态间血小板膜糖蛋白CD62p,CD63, CD41a表达量都没有显著性差异;而先兆子痈孕妇组静息态与活化态血小板膜糖蛋白CD62p,CD63表达量均较正常 孕妇组显著增高，CD41a表达无显著性差异。结论 先兆子痈产妇体内血小板膜糖蛋白CD62p,CD63表达处于较高 水平，提示血小板处于活化状态。 关键词:流式细胞术;血小板活化;先兆子痛 【中图分类号〕R446.11 文〔献标志码了A 文〔章编号〕1009-6213(2005)02-068-03 TheDetectionofPlateleteActivitybyFlowCytometryMethodintheGravidawith Pre-eclampsia YIZheng-shan,MENGFan-yi,FENGRu,LIURong,SONGLan-lin,ZHOUShu-yun (HematologicalDepartmentofNanfangHospitaloftheFirstMillitaryCollege,Guangzhou510515,China) Abstract:O讨ectiveTheactivationofplateletewasdetectedbyusingtheflowcytometrymethodte- chonolygy.ToexploretheclinicsignificanceofthevariationoftheplateleteglycoproteinCD41a,CD62p,CD 63expressioninstaticandactivatedinthegravidawithpre-eclampsia.MethodsWeusedno-washedimmu- nitylabellingtechniqueinbloodofsubjectswhichincluded16gravidawithpre-eclampsiaand15normal pregnanciesand20healthpersons.Thefluorescencelabblledmonoantibodiesofplateleteglycoproteinincluded CD41a，CD62p,CD63werereactedwithstaticandactivatedplatelete.Theexpressionofmonoantinbody weredectectedbyflowcytometry.ResultsTheaveragestaticandactivatedplateleteglycoproteinCD41a,CD 62p，CD63expressionlevelsinthegravidawithpre-eclampsiawereapparentlyhigherthanthoseofthenormal pregnancy(P0.01);whilethecomparisonbetweenthelatterandthecontrolgroupdidnotrevealsignificant difference(P0.05).Conclusion HighlevelsofplateleteglycoproteinCD62pandCD63expressionare presentingravidawithpre-eclampsia.Itisinfertheactivationofplateletwerepresentinthe