肺癌患者1433σ基因启动子甲基化与其mRNA表达水平的关系.docVIP

肺癌患者14-3-3σ基因启动子甲基化与其mRNA表达水平的关系 作者:余宗涛,袁亚莉,范立新 【关键词】 肺癌;14-3-3Σ基因;启动子;甲基化;表达 　　[摘要]　　目的:探讨肺癌患者14-3-3Σ基因启动子CpG岛甲基化状况与其mRNA表达水平的关系。方法:用实时定量PCR检测14-3-3Σ基因mRNA在肺癌组织、正常肺组织及肺癌细胞株A549中的表达；用甲基化特异性PCR检测这些组织、细胞中14-3-3Σ启动子区CpG岛甲基化状态。结果：14-3-3Σ基因启动子在肺癌组织和正常肺组织中发生甲基化的频率分别为51.11%(23/45)和17.39%(4/23)（Χ2=7.229,P=0.007）。14-3-3Σ mRNA在正常组织中全部表达，在肺癌组织中表达缺失率为15.56%(7/45)，且表达量低于正常肺组织（t=10.309,P0.001），不同肿瘤病理分型(SCLC、NSCLC)之间14-3-3Σ mRNA有显著差异(t=5.256,P0.023)，不同年龄、性别、肿瘤大小之间无显著差异（P0.05）。14-3-3Σ启动子甲基化与其表达量下降密切相关（r=0.48,P=0.04）.结论：在肺癌中14-3-3Σ启动子甲基化与其mRNA表达下调或缺失有密切关系，在肺癌发生中14-3-3Σ异常甲基化起一定作用。 　　[关键词]　　肺癌;14-3-3Σ基因;启动子;甲基化;表达 　　The Relationship Between Promoter Hypermethylation of 14-3-3Σ Gene and Transcription of 14-3-3Σ in Lung Carcinoma　　 　　YU Zong-tao,YUAN Ya-li,FAN Li-xin,JIAO Hai-chun,QIN Yong 　　(Department of Science and Biological Technology,Nanhua University,Hengyang，Hunan 421001，China) 　　Abstract:Objective To investigate the relationship between the promoter methylation and the mRNA expression of 14-3-3Σ gene in lung carcinoma.Methods The promoter methylation status of 14-3-3Σ in 45 lung carcinoma tissues, 23 normal tissues and A549 cell lines was detected by methylation-specific PCR, its mRNA expression level was measured by real-time reverse transcription polymerase chain reaction.Results The frequency of promoter methylation of 14-3-3Σ gene was 51.11%(23/45) in lung carcinoma and 17.39%(4/23) in normal tissues（Χ2=7.229,P=0.007）,respectively.14-3-3Σ mRNA was all dectected in 23 normal tissues and there was no expression of 14-3-3Σ in 15.56%(7/45) of lung carcinoma. The mRNA expression of 14-3-3Σ was significantly lower in lung carcinoma tissues than in normal tissues（t=10.309,P0.001）. However, there was no correlation of 14-3-3Σ mRNA expression with clinical data, such as sex, age, tumor size (P 0.05),but there was a significant correlation between pathological type(SCLC、NSCLC,t=5.256,P＝0.023).In 23 patients with the aberrant promoter methylation