高浓度乙酸注射治疗兔VX2肝肿瘤的实验研究.docVIP

高浓度乙酸注射治疗兔 VX2 肝肿瘤的实验研究 高浓度乙酸注射治疗兔 VX2 肝肿瘤的实验研究 孙志远 　丁仕义 　黄学全 　　【摘要】　目的 　利用兔 V X2 肝移植瘤模型探讨高浓度乙酸注射治疗肝肿瘤的价值 ,并探索注射 治疗肝肿瘤的最适乙酸浓度。方法 　用移植法建立兔 V X2 肝肿瘤模型 ,将 40 只携瘤兔随机分为 A 、 B 、C 、D 四组 。术前以 C T 测量肿瘤直径 。开腹向 A 、B 、C 、D 组肝肿瘤内分别注入 50 % 、75 % 、100 % 乙酸和生理盐水 ,48 h 后重复注射一次 ,术后一周处死兔子取肝脏观测比较各组肿瘤体积改变情况 及坏死灶周围新生灶情况 , 并取肿瘤组织制作石蜡切片 ( H E 染色) , 镜下观察肿瘤组织坏死情况。 结果 　注射后一周各实验组肿瘤直径均减小 ,对照组肿瘤直径明显增大 。100 %乙酸组 10 例仅 1 例 在坏死灶周围发现新生灶 ,而 50 %乙酸组发现 5 例 ,75 %乙酸组发现 7 例 。镜下显示 100 %乙酸组肿 瘤中未坏死的肿瘤细胞团较 75 %和 50 %乙酸组少见 。结论 　高浓度的乙酸对兔 V X2 肝肿瘤的灭活 效果显著 ;其中 100 %的乙酸效果最佳 。 　　【关键词】　肝肿瘤 　　乙酸 　　疾病模型 ,动物 　　兔 Acetic acid injection f or the treatment of VX2 l iver tumor by diff erent concentration of acetic acid : an ex2 perimental study in ra bbit 　S un Z hi y ua n , D i n g S hi y i , H u an g X uequa n . De p a rt me nt o f R a d i ol o g y , S out h w es t H os p i t al , T hi r d M i ni t a r y M e d ic al U ni ve rs i t y , Chon g qi n g 400038 , Chi na 　　【Abstract 】　Objective 　To st udy t he val ue of acetic acid injectio n fo r t he t reat ment of liver t umo r , and explo re t he best co ncent ratio n of acetic acid. Methods 　40 rabbit s wit h impla nted V X2 liv2 er t umo r were rando mly were divided into 4 gro up s. Befo re t he t reat ment , t he diameter of t he t umo r s wa s acquired by C T. The a bdo men wa s opened , and 50 % , 75 % , 100 % acetic acid o r p hysiolo gic sa2 line wa s injected into t he t umo r s of A , B , C , D gro up s , re spectively. Af ter 48 ho ur s acetic acid were injected o nce at t he sa me do sage . Af ter a week t he ra bbit s were killed and t he liver s were re sected. The t umo r sdiameter were deter mi ned and t he ca se s of t he rena scent t umo r s were co unted in each gro up . So me t umo r ti ssue s were sampled make p araffin sectio n ( H E staining) a nd o bserve t he necro si s of t umo r ti ssue wit h micro scop e. Results 　1 week af ter i njectio n , t he average dia meter of t umo