《Argyrophilic nucleolar organizer region in MIB1 positive cells in nonsmall cell lung cancerclinicopathological signiifcance and sur vival》.pdfVIP

Cancer Biol Med 2014;11:264-269. doi: 10.7497/j.issn.2095-3941.2014.04.005 ORIGINAL A RTICLE Argyrophilic nucleolar organizer region in MIB-1 positive cells in non-small cell lung cancer: clinicopathological significance and survival 1 2 2 Dmitriy Sergeevich Kobyakov , Ashot Merudzhanovich Avdalyan , Aleksandr Fedorovich Lazarev , Elena Leonidovna 3 3 Lushnikova , Lev Moiseevich Nepomnyashchikh 1Budget Institution, Kogalym City Hospital, Kogalym 628484, Russia; 2 Laboratory of Molecular Diagnostics of Altai Branch of Russian N.N. Blokhin Cancer Research Center, Barnaul 656049, Russia; 3 Federal State Budget Institution Research Institute of Regional Pathology and Pathomorphology of Siberian Branch of Russian Academy of Medical Sciences, Novosibirsk 630117, Russia AbstrAct Objective: To evaluate the relation between argyrophilic nucleolar organizer region (AgNOR)-associated proteins and clinicopathological parameters and survival in non-small-cell lung cancer (NSCLC). Methods: A total of 207 surgical specimens diagnosed as NSCLC were included in this study. Double-staining procedures were performed using antigen Ki-67 (clone MIB-1) and silver nitrate by immunohistochemical and AgNOR-staining methods. Results: The AgNOR area in MIB-1-positive cells of NSCLC is related to clinicopathological parameters under the TNM (tumor, node, and metastasis) system. The survival of patients with small AgNOR area in MIB-1-positive cells is better than that of patients with large AgNOR area. Molecular, biological (AgNOR area in MIB-1-positive cells),