_氨基丁酸受体活化增强神经型一氧化氮合酶磷酸化对缺血性脑损伤的保护作用.pdf

山西医药杂志2010 年10 第39 卷第10 期上半 Sh anx i M ed J, October 2010, Vol. 39, No. 10 the Fir t # 899 # # # C-氨基丁酸受体活化增强神经型一氧化氮合酶 磷酸化对缺血性脑损伤的保护作用 徐州医学院机能实验中心( 22 1002) 周 翠 张光毅 = C- ( GA BA) ( nN O S) , SD , GABAA GABA B ; , nN OS 1 d nNO S( Ser 847) , 1 d nN OS ( Ser847) GABA GA BA nN OS( Ser 847) A B = ; , GABA ; Neuroprotection of GABA receptor agonists via enhancing nNOS(Ser847 ) phosphorylation in cerebral ischemia ZH O U Cui , ZH A N G Guang-y i. Exp er iment Center of Functional Science , X u hou M edical Colleg e, J iang su 22 1002, China = Abstract Objective T hi tudy i to inve tig ate the r ole of enhancement of GABA r eceptor act ivity on the pho phory latio n of nNO S and the po ible molecular mechani m of inhibitory neur otr an mi io n during brain i- chemia. Methods A dult male Sprague- Daw ley rat w ere u ed t o induce four- ve el occlu io n cerebral i chemia model , combination of GA BA recept or ag oni t ( mu cimol and baclofen) w a u ed. And w e u ed immunopr ecip-i tatio n and w e ter n blot ting to ob erve nN O S( Ser847) pho phory lat ion. Results Combinatio n of two ag oni t could increa e the int eraction of nNO S w it h PSD95 and nNO S( Ser847) pho phorylatio n at 1 day reperfu ion. onclusion Co- application of GA BA r eceptor ago ni t could ex ert it neur oprotect ive effect on cer ebr al i chemia by increa - ing nN OS ( Ser 847) pho phorylation. = Key words Brain i chemia; Recptor , GA BA ; Nitric-oxide yntha e NO , GABA , C- ( GABA ) , GABA