树脂相分光光度法测定生物样中微量铁(嘎日迪).docVIP

树脂相光, 200040） 摘 要 ： 铁??）与2,2′—联吡啶pH值5左右HAc-NH4Ac缓冲溶液强酸性阳离子交换树脂，相??)。最大吸收波长λmax=522nm，表观摩尔吸光率为9.8×104L?mol-1?cm-1，比水相中直接分光光度法测定Fe(??)的摩尔吸光率提高11倍多。铁??）的含量在0.2~20mg?L-1范围内符合Beer定律。用此法测定了红豆、黑豆、荞麦和莜麦中的微量铁关 键 词：2,2′—联吡啶铁铁中图分类号： 文献标识码：Resin-Phase Spectrometry Analysis for Trace Iron in Biological Sample SHI Dong-hui1 FENG Shan2 (1:Paramedical School, LIDA Professional College, Shang Hai, 201690 2:HUXI Subsidiary Company, Medicine Co. Ltd. Shang Hai, 200040) Abstract: A new method for determination of trace iron by resin phase spectrometry was studied in this paper. Fe(Ⅱ) and 2,2′- bipyridine can form a colored complex cation in HAc-NH4Ac buffer solution at pH 5.0 and the colored complex cation was adsorpted by strong acidic cation changeable resin, according to this, trace Fe(Ⅱ) can be determined by resin phase spectrometry. The maximum wavelength is at 522nm. The apparent molar absorbance is 9.8×104L?mol-1?cm-1, which is ten times sensitive than that of direct water phase spectrometry. The Beer’s law was obeyed when Fe(Ⅱ) was in the range of 0.2~20mg?L-1. the result was applied to determine the trace iron in small red beans、black soy beans、buckwheat and Avena chinensis with relatively higher sensitivity and precision. The relative standard deviations and recoveries of which are 1.9%~3.5% and 97.8%~103% respectively. Keywords: Resin-Phase Spectrometry; 2,2′—bipyridine; Iron 树脂相（固相）分光光度法报道较多[1~7]。此法的特点是分离、富集和显色等同时进行，所以灵敏度高、选择性好、操作简单、快速。2,2′—联吡啶用铁??）与2,2′—联吡啶pH值5左右Ac~NH4Ac缓冲溶液强酸性阳离子交换树脂，相?g-1、78.59μg?g-1、45.90μg?g-1、37.34μg?g-1，此结果与原子吸收分光光度收稿日期： 作者简介：石东辉（1967-），女，讲师，主要从事工作护理及医学，E-mail: 测定结果基本一致 。 1 实验部分.1 仪器与试剂722型分光光度计(上海第三分析仪器厂)Z-8000型塞曼偏光原子吸收分光光度计(日本日立公司)?? )的标准储备溶液：准确称取硫酸铁铵?L-1，使用时稀释为10.0mg?L-1的工作溶液。盐酸羟胺(NH2OH·HCl)溶液：10% 。2,2′—联吡啶C10H8N2）溶液：10% 。pH=5.4的HAc~NH4Ac缓冲溶液：称取39gNH4Ac溶于水中，加6 mL冰醋酸，稀释至500mL：H2O2：30% 。732型强酸性阳离子交换树脂，将其先用二次蒸馏水漂洗，再用mol·L-1的HCl浸泡24h然后用二次蒸馏水洗至中性，自然晾干备用.2 实验方法 准确移取一定量的Fe (?? )工作溶液或试液于25mL容量瓶中，依次加入10%胺(NH2OH·HCl)溶液1.0