移植前基因诊断的卵裂球活检操作是否影响早期血清贝塔hCG水平.pdf

ORIGINAL ARTICLE doi: 10.5653/cerm.201 pISSN 2233-8233 · eISSN 2233-8241 Clin Exp Reprod Med 2011;38(1):31-36 Does blastomere biopsy in preimplantation genetic diagnosis affect early serum β-hCG levels? 1 1 1 2 2 1 1 Yeon Jean Cho , Jin Yeong Kim , In Ok Song , Hyung Song Lee , Chun Kyu Lim , Mi Kyoung Koong , Inn Soo Kang 1 2 Department of Obstetrics and Gynecology, Laboratory of Reproductive Biology and Infertility, Cheil General Hospital Women’s Healthcare Center, Kwandong University College of Medicine, Seoul, Korea Objective: To determine whether the serum β-human chorionic gonadotropin (hCG) profile following preimplantation genetic diagnosis (PGD) is lower than that of intracytoplasmic sperm injection (ICSI) cycles. Methods: A total of 129 PGD cycles and 1,161 age-matched ICSI cycles, which resulted in pregnancy (serum β-hCG ≥ 5 mIU/mL) on post-ovu- lation day (POD) 12 were included. We compared the mean serum β-hCG levels on POD 12, 14, 21, and 28, doubling time of serum hCG, and created a cut-off value for predicting a singleton pregnancy in each group. Results: The mean serum β-hCG concentration of the PGD group was significantly lower than that of the control group on POD 12, 14, and 21. The doubling time of serum β-hCG at each time interval showed no significant difference. The cut-off-value of serum β-hCG for predicting a single viable pregnancy was 32.5 mIU/mL on POD 12 and 113.5 mIU/mL on POD 14 for the PGD group, which was lower than that for the con- trol group. Conclusion: Blastomere biopsy may decrease the β-hCG-producing activity of the trophoblasts, especially in early pregnancy. Setting a lower cut-off value of serum β-hCG for predicting pregnancy outcomes in PGD may be needed. Keywords: Preimplantation Genetic Diagnosis