紫花苜蓿SANDITI愈伤组织诱导和分化的研究.pdf

2007, 11 ( 1): 77- 81 JournalofH ebeiAgriculturalSciences SA DITI 1 2 肖荷霞, 王 瑛 ( 1, 061001; 2, 071002) : 利用紫花苜蓿 SA DIT I无菌苗的下胚轴茎和叶 3种外植体, 研究了在附加 同激素浓 度的MS培养基上诱导愈伤组织的效果结果表明: SA DIT 最佳外植体为下胚轴, 最佳下胚轴 愈伤组织诱导培养基为MS+ 2 4D 20mg/L+ 6BA 05mg/L15d继代 1次, 或适当降低 2 4D 浓度提高 6BA 或KT浓度, 可以降低愈伤组织的褐化率用源于下胚轴的愈伤组织在 SH + 2 4D 10mg/L+ 6BA 05mg/L+ CH 2000mg/L培养基中进行悬浮培养, 每 5d继代 1次, 1 个 月可以建立良好的胚性悬浮系, 并伴有体细胞胚胎的发生愈伤组织在 6BA 05mg/L AA 01mg/LGA 10mg/LYE 250mg/LCH 250mg/L的MS培养基上, 可获得较高的分化 3 率 : 紫花苜蓿; 愈伤组织; 诱导; 再生; 体细胞胚胎 : Q9446 : A : 2007) Inducing andRegeneration ofCallusofSANDITIM ed icago Sativa L 1 2 XIAO H exia, WA G Y ing ( 1Cangzhou Institute ofAgriculture and Forestry Science Research, Cangzhou 061001, China; 2College ofL ife Science, HebeiUniversity, Baoding 071002, China) Abstract: Different explants of alfalfa (M ed icag o Sa tiva L) SA DITI seedlingwere cultured on themediaof dif ferent phytohormone combinationsThe results indicated thatMS+ 2 4D 20mg/L + 6BA 05mg/Lmediumwas the best to induce callusw ith the highest induction frequency from hypocotylReducing the concentrations of auxin and raising the concentrations of kinin was benefit for the reducing of brow ing rateThe calli from hypocotylswere cultured in liquidm edium SH + 2 4D 10mg/L+ 6BA 05mg/L+ 2 000mg/L, somatic embryos appesredThe higher regeneration rate can be get from MS medium w ith 6BA 05mg/L, AA 01mg/L and GA 10mg/L 3 Keywords: M ed icag o S ativa L; Callus; Induction; Regeneration; Somatic embryo (A fl afl a ) , , ,