干旱胁迫对花荚期大豆叶片保护酶活性和膜脂过氧化作用的影响.pdfVIP

安徽农业科学。JournalofAnhui 责任编辑朱永和责任校对朱永和 Agri．Sci．2006，34(8)：1528-1530 干旱胁迫对花荚期大豆叶片保护酶活性和膜脂过氧化作用的影响 王启明，郑爱珍，吴诗光 (商丘师范学院生物系，河南商丘476000) 摘要以2个不同耐旱性大豆品种为材料，研究了干旱胁迫对大豆花荚期叶片保护酶活性和膜脂过氧化作用的影响。结果表明：随 POD活性高于SOD、CAT活性。 较低的质膜透性和MDA含量，同时具有较高的SOD、CAT和POD活性；重度干旱／Ib迫T 关键词大豆；干旱胁迫；保护酶活性；膜脂过氧化 中图分类号$643．7文献标识码A 文章编号0517—6611(2006)08—1528—03 WANG et Teachers 476000) Qi-ming ofBiology，ShangqiuCollege，Shangqiu，Henan al(Department membrane inleafof AbstractTheeffectsof onthe and drought—stressprotectiveenzymeactivity lipidperoxidationsoybean andtheir withthetoleranceto werestudiedwitIltwodifferent cuhivars．Theresultshowedthatwith period relationship drought drought—tolerant contentsofMDAinereased activitiesofSOD，PODand the of of membraneand strengthdrought．stress．permeabilityplasmas gradually，and rateof of membraneand were in CATroseatfirstandloweredlater．Muchlower permeabilityplasma appeared the oneshad SOD，CAT caltivarsthanthosein cuhivarsunder higher drought．tolerant drought—sensitive drought．stress．Anddrought—tolerant ofPODwas thanthoseofSODandofCAT． andPODactivities．Insevere conditions，theactivity drought higher words of enzvme；Membrane Key Soybean；Drought—stress；Activityprotective lipidperoxidation 在逆境条件下植物的膜脂过氧化反应和保护酶系统包 酶活力单位；POD活性测定采用愈创木酚法旧，以每分钟内 括超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶nm变化0．01为1个酶活单位。所测指标均选用大豆茎尖 A470