苹果果实发酵液对草莓防御酶系活性及抗病性的影响.pdfVIP

中国农学通报 2010,26 16 :250-255 Chinese Agricultural Science Bulletin 苹果果实发酵液对草莓防御酶系活性及抗病性的影响 1 1 1 2 2 1 2 王荣娟 ，戚亚平 ，宋备舟 ，黄 彬 ，王 雷 ，姚允聪 ，姬谦龙 1 2 （北京农学院植物科学技术学院，北京102206；北京农学院生物技术系，北京102206） 摘 要：探讨在有机栽培方式下，苹果果实发酵液诱导草莓抗灰霉病（Botrytis cinerea ）的作用。用4 个处 理喷施草莓，5 天后接种灰霉病病菌。接菌6 天后统计不同处理的病斑数，同时测定过氧化物酶（POD ）、 多酚氧化酶 PPO 及β-1,3- 葡聚糖酶在接种后12 天内的动态变化。Tr2 处理后，草莓的抗病指数达到 34.6% ，显著高于对照，其他处理差异不显著。喷施Tr 1、Tr2、Tr3 可使POD 、PPO 酶活性高于对照，β-1,3- 葡聚糖酶活性高峰出现较对照早。苹果果实发酵液100倍稀释液和0.02 mol/mL 水杨酸混合喷施可诱 导草莓对灰霉病产生抗性。这对草莓有机生产中草莓灰霉病的防治提供了新途径。 关键词：苹果果实发酵液；草莓；灰霉病；水杨酸；抗病性 中图分类号：S432.4 文献标志码：A 论文编号：2010-0873 Influences of Apple Fruit Broth on Activities of Defense Enzymes and Disease Resistance on Strawberry 1 1 1 2 2 1 2 Wang Rongjuan , Qi Yaping , Song Beizhou , Huang Bin , Wang Lei , Yao Yuncong , Ji Qianlong 1 2 College of Plant Science and Technology in BUA, Beijing 102206; Department of Biotechnology in BUA, Beijing 102206 Abstract: This paper explored the effect of the apple fruit broth on inducing resistance of strawberries to Botrytis cinerea under the organic cultivation. We sprayed strawberries with four different treatments. Then 5 days later, the strawberries were inoculated with Botrytis cinerea. 6 days after inoculating, the numbers of necrotic lesions were counted and activities of peroxidase, polyphenol oxidase and β-1, 3-glucanase were assaied. The results showed that the Tr2 could not only induce resistance of the strawberries on the Botrytis cinerea, its index of disease resistance reached 34.6% , which was significantly higher than the control. Difference between other treatments was not significant. Sprayed with Tr1, Tr2, Tr3, the activities of POD, PPO were higher than the control, activity of β-1, 3-glucanase appeared earlier than the control did. The mixed liquor of 100-fold diluted apple fruit broth and 0.02mol/mL salicylic acid could induce desease resistance of the strawberry to Botrytis cinerea. The research may pr