颐脑解郁方对海马神经元缺氧%2f复氧损伤突触素表达的影响.pdfVIP

颐脑解郁方对海马神经元缺氧/复氧损伤突触素 表达的影响 朱明瑾1唐启盛2李小黎2杨歆科2李冬梅2 1北京中医药大学，北京1000292北京中医药大学第三附属医院，北京 100029 摘要：目的：探讨颐脑解郁方对缺氧/复氧条件下离体海马神经元突触素表达的影响。方法：以正常大鼠、 经颐脑解郁方及尼奠地平灌胃大鼠血清为媒介，制备正常及缺氧/复氧损伤后海马组织星形胶质细胞条件培养液。 提取乳鼠海马神经元并进行体外培养，根据所应用干预条件培养液的不同随机将神经元分为正常组、模型组、 中药组、西药组4组。采用MTT法观察缺氧3h(is-3h)及复氧24h(rp-24h)时点海马神经元活性并计算细胞存活 率，同时应用免疫细胞化学方法测定海马神经元突触素的表达。结果：is-3h时点模型组神经元活性、存活率及突 触素表达均较正常组明显降低(P＜0.01)，与中药组比较无明显差异；rp-24h时点中药组上述指标均较模型组明 显升高(P＜0.01)。结论：颐脑解郁方可明显促进缺氧/复氧损伤后海马神经元突触素表达的增强，该方可能通过 有效增加神经元突触密度而对缺血/再灌注损伤神经功能恢复发挥明显改善作用。 关键词：颐脑解郁方；缺氧/复氧；海马神经元；突触素 EffectsofYinaoJieyuPrescriptiononthesynaptophysinexpressionofthedamaged hippocampaineuronsinducedbysimulatedhypoxia/reoxygenationinvitro ZHUMing-jin1TANGQi-sheng2LIXiao-li2YANGXin-ke2LIDong-mei2 1BeijingUniversityofChineseMedicine,Beijing100029,China 2 The Third Affiliated Hospital of Beijing University of ChineseMedicine,Beijing100029,China Abstract:Objective:ToexploretheeffectofYinaoJieyuprescriptiononthesynaptophysinexpressionofthedamaged hippocampalneuronsinducedbysimulatedhypoxia/reoxygenationinvitro.Methods:Fourdifferentastrocyteconditioned mediumsweremadebasedontheapplicationoftheinterventionoftheserumswhichcontaineddifferentmedicines.Rat hippocampalneuronswereculturedanddividedinto4groupsaccordingtothesedifferentastrocyteconditionedmediums includingnormalgroup(N),modelgroup(M),Chinesemedicinegroup(CM)andwesternmedicinegroup(WM).Theactivityand viabilityofthedamagedneuronsbothat3hoursafterhypoxiaand24hoursafterreoxygenationwereevaluatedbycolorimetric MTTassay.Theexpressionofsynaptophysininhippocampalneuronswasmeasuredbythemethodofimmunocytochemistry. Results:Theactivity,viabilityandsynaptophysinexpressioninMwereallsignificantlylowerthanN(P＜0.01),andtherewas nodifferenceinCMat3hoursafterhypoxia.Andat24hoursafterreoxygenation,theactivity,viabilityandsynaptophysin expressioninCMweremuchhigherthanM(P＜0.0