喜树碱对番茄灰霉病菌的病理反应.pdfVIP

30(1)143-148 中国生物防治学报 Chinese Journal of Biological Control 2014 年 2 月 喜树碱对番茄灰霉病菌的病理反应 1,2 2 2 2 1* 毛胜凤 ，林海萍 ，陈安良 ，张立钦 ，叶建仁 （1. 南京林业大学森林资源与环境学院，南京 210037 ；2. 浙江农林大学林业与生物技术学院，临安 311300) 摘要：以番茄灰霉病菌为供试菌种，采用电导率法、吸光度法和电镜分析法对经过喜树碱（CPT ）处理的 番茄灰霉病菌进行病理反应研究。试验结果表明经 200 mg/L CPT 溶液处理后，番茄灰霉病菌菌丝细胞膜 的通透性增加了 30.7% ，细胞外蛋白质浓度提高了 13.5%。番茄灰霉病菌经 CPT 溶液处理后，还原糖和可 溶性蛋白的含量随着处理时间的增长先升高后降低最后趋于平缓，在 0～1 h 之间增长最快。CPT 处理后的 番茄灰霉病菌的菌丝畸形，出现膨大或缢缩，菌丝生长点缢缩，细胞中的空腔增多，线粒体增多且线粒体 嵴变得模糊，细胞核有降解的现象。 关 键 词：喜树碱；番茄灰霉病菌；细胞膜通透性；内含物；电镜 中图分类号：S476 文献标识码：A 文章编号：1005-9261 (2014)01-0143-06 Antifungal Reaction of Botrytis cinerea on Camptothecin 1,2 2 2 2 1* MAO Shengfeng , LIN Haiping , CHEN Anliang , ZHANG Liqin , YE Jianren (1. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037; 2. School of Forestry and Bio-technology, Zhejiang Agriculture and Forestry University, Lin’an 311300, China) Abstract: The mechanism of camptothecin (CPT) against Botrytis cinerea was studied by conductivity method, UV spectrophotometry and electron microscopy in this paper. The results showed that the membrane permeability of B . cinerea increased 30.7%, extracellular protein concentration increased 13.5% after 200 mg/L CPT solution treatment. The sugar and soluble protein content of B . cinerea increased firstly, especially in 0-1 h and then decreased with the growth of the processing time, and finally tended to flatten after CPT solution treatment. The hyphae malformed with swollen or constriction and the growth point of mycelial constricted after CPT treatment. At the same time, the cell cavities and mitochondri