低强度超声对早孕大鼠药物流产后子宫组织中内皮素-1和一氧化氮的影响.pdfVIP

·100· doi 10.3969/j.issn. 1673-4254.2014.01.22 J South Med Univ, 2014, 34(1): 100-102 基础研究 低强度超声对早孕大鼠药物流产后子宫组织中内皮素低强度超声对早孕大鼠药物流产后子宫组织中内皮素-- 11和一氧和一氧 化氮的影化氮的影响响 张艳霞，郭菊芳，林 川，陆 露，李成志 重庆医科大学生物医学工程学院//省部共建超声医学工程国家重点实验室//超声医学工程重庆市重点实验室， 重庆 400016 摘要：目的观察低强度超声对早孕大鼠药物流产后子宫组织内皮素- 1（ET- 1）和一氧化氮（NO ）的影响。方法雌性早孕大鼠30 只，随机分成治疗组和对照组，采用米非司酮和米索前列醇造成流产模型，治疗组给予低强度超声治疗30 min/d ，共5 d ，对照组 采用与治疗组相同的操作方法进行治疗，但仪器无功率输出。5 d 后取子宫组织制成匀浆，分别用酶联免疫法和化学测试法检 测ET- 1和NO 的含量。结果治疗组子宫组织中ET- 1含量明显高于对照组（P0.05）；NO 含量差异无显著性（P0.05）。结论低 强度超声可能通过增加子宫组织中内皮素-1含量，改变ET- 1与NO 的动态平衡，从而促进子宫平滑肌的收缩。 关键词：低强度超声；早孕；药物流产；子宫；一氧化氮；内皮素-1 Effect of low-intensity ultrasound on endothelin- 1 and nitrogen monoxidum in uterine tis- sues of SD rats following abortion ZHANG Yanxia, GUO Jufang, LIN Chuan, LU Lu, LI Chengzhi State Key Laboratory of Ultrasound Engineering in Medicine Co- founded by Chongqing and the Ministry of Science and Technology, Chongqing Key laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing 400016, China Abstract: Objective To investigate the effect of low- intensity ultrasound on the contents of endothelin (ET- 1) and nitrogen monoxidum (NO) in uterine tissues of SD rats after abortion. Methods Thirty female pregnant rats were randomly divided into treatment group and control group and received mifepristone and misoprostol to induce abortion. The rats in the treatment group were treated by low- intensity ultrasound for 30 min/day for 5 consecutive days, and those in the control group received sham treatment. The uterine tissue was then taken and homogenized for measurement of ET- 1 and NO contents using enzyme- linked immunosorbent assay and chemical testing. Results ET- 1 content in the uterine tissues was significantly higher in th