低温冷冻对人精子顶体超微结构的影响.pdfVIP

·114· 中潮优生与遗传杂志2008年第16卷第3期 低温冷冻对人精子顶体超微结构的影响 蒲军1。陈在贤2 (1．重庆医科太学公共卫生学院2．璧庆医科大学附属一院泌尿科，重庆400016) 摘要：骂的评价低温冷冻麓不同冷冻方法对人精予颁铬超微结构的影响。方法将人的精子分捌用速冻法筑缓冻 潦冷冻1年。在冷冻前后分别用电镜观察精子顶体的超微结构，比较冷冻前后及幂同冷冻方法间顶体超微结构的变化。结 果顶体超徽结构相对正常(异常)的精子在冷冻后显著减少(增加)；速冻法岛缓壤法柱比，顶体起微结构相时正常 (秀掌)戆糖子减多’(罐加)更显著。结论诋温冷冻将对人精子磺俸的超微鐾魏造成蓣害；缓壤法靖人靖子礓蘩起微结 构的损害较轻。 关键词：冷冻保存；精子；顶体；超微结构 孛雪分类号：R321；l 文献标谖码：A 文章续号：1006—95弘《2008}03-0114—02 of Theeffectof ontheultmstructureofacrosomehuman cryopreservation School First Medwd ofPublic ofthe 400016，China) Health，2。DepartmentofUrology Hospital，ChongqingUniversity，Chongqing evaluateeffectsof different methodsontheultrastructureofaerosomeof Abstract：Objective：Tocryop咒se球8ti黼andfreezing forl withthe methodand slow human human the method，the sperm．Methods：Tocryopreservatespermyear quickfreezing freezing ultrastructureofacmsomewillbeobservedelectron beforeandafter and the oftheultrastructure by microscope freezingcomparechange ofacr璐omeofhumanbeforeand different findoutthat with methods．Results：We sperm after翻葫醒andfreezing spermsapproximate offlcrosome after and with normal normal(abnormal)uhrastrueturedecreased(increased)significantlyfl雠zingspermsapproximate after methodthanthose slow ofacrosome after (abnormal)ultrastrueturedecreased(increased)significantlyquickfreezing freezing