Design of a Highly Selective Quenched Activity-Based Probe外文翻译.pdfVIP

Article /JACS Design of a Highly Selective Quenched Activity-Based Probe and Its Application in Dual Color Imaging Studies of Cathepsin S Activity Localization Kristina Oresic Bender,† Leslie Ofori,† Wouter A. van der Linden,† Elliot D. Mock,† Gopal K. Datta,∥ Somenath Chowdhury,∥ Hao Li,§ Ehud Segal,† Mateo Sanchez Lopez,† Jonathan A. Ellman, ∥,# Carl G. Figdor,⊥ Matthew Bogyo,*,†,‡,§ and Martijn Verdoes*,†,⊥ †Departments of Pathology, ‡Microbiology and Immunology, and §Chemical and Systems Biology, Stanford University School of Medicine, Stanford, California 94305, United States ∥Department of Chemistry, University of California-Berkeley, Berkeley, California 94720, United States ⊥Department of Tumor Immunology, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, 6500 HB Nijmegen, The Netherlands S *Supporting Information ABSTRACT: The cysteine cathepsins are a group of 11 proteases whose function was originally believed to be the degradation of endocytosed material with a high degree of redundancy. However, it has become clear that these enzymes are also important regulators of both health and disease. Thus, selective tools that can discriminate between members of this highly related class of enzymes will be critical to further delineate the unique biological functions of individual cathepsins. Here we present the design and synthesis of a near-infrared quenched activity-based probe (qABP) that selectively targets cathepsin S which is highly expressed in immune cells. Importantly, this high degree of selectivity is retaine