《Two-photon laser scanning fluorescence microscopy using photonic crystal fiber》.pdf

Journal of Biomedical Optics 9(5), 922–927 (September/October 2004) Two-photon laser scanning fluorescence microscopy using photonic crystal fiber Gail McConnell Abstract. We report the application of a simple yet powerful modular University of Strathclyde pulse compression system based on photonic crystal fibers that im- Centre for Biophotonics proves on incumbent two-photon laser scanning fluorescence micros- SIBS, 27 Taylor Street Glasgow G4 0NR, Scotland copy techniques. This system provides more than a sevenfold increase in fluorescence yield when compared with a commercial two-photon Erling Riis microscopy system. From this, we infer pulses of IR radiaton of less University of Strathclyde than 35 fs duration reaching the sample. © 2004 Society of Photo-Optical In- Department of Physics strumentation Engineers. [DOI: 10.1117/1.1778734] John Anderson Building Glasgow G4 0NG, Scotland Keywords: multiphoton; microscopy; fluorescence; ultrafast; nonlinear. E-mail: g.mcconnell@strath.ac.uk Paper 03110 received Sep. 3, 2003; revised manuscript received Jan. 8, 2004, and Feb. 5, 2004; accepted for publication Feb. 16, 2004. 1 Introduction made to precompensate for dispersion by including prisms in Multiphoton laser scanning microscopy MPLSM is a well- a home-built laser resonator, delivering pulse