癌性腹水中的肿瘤相关淋巴细胞对小肠Cajal间质细胞损伤的实验研究.pdfVIP

·316· ·基础研究· 癌性腹水中的肿瘤相关淋巴细胞对小肠Cajal间质细胞 损伤的实验研究 金晓明 孟宏学 张守臣 郑宏群 【摘要】 目的观察癌性腹水中淋巴细胞对小肠Cajal间质细胞的影响，探讨癌性腹水所产生的 胃肠蠕动功能障碍的病理生理学基础。方法建立癌性腹水模型，进行腹水成分的鉴定及小肠Cajal间 质细胞的病理学、超微病理学、c—kit免疫荧光及凋亡指标的检测。结果癌性腹水中的淋巴细胞以T淋 巴细胞为主，这些肿瘤相关的淋巴细胞可导致小肠Cajal间质细胞的损伤、凋亡。结论肿瘤相关淋巴 细胞可能造成小肠Cajal间质的损伤，是癌性腹水所产生的胃肠蠕动功能障碍的病理生理学基础。 【关键词】 癌性腹水；小肠Cajal间质细胞；肿瘤相关淋巴细胞；凋亡 on of for interstitialcellsofsmallintestinalcancerousas- tulnor-infiltratinglymphocytesCajal Damage cites JIN Xiaoming+，MENGHongxue，ZHANGShouchen，ZHENGHongqun．。DepartmentofPathology， HarbinMedical 150081，China University，Harbin author：JIN CorrespondingXiaoming，Email：jinxm55@163．eom Observedthe onsmallintestinein— cancerousascites 【Abstract】Objective lymphocyte’Simpact Cajal terstitialcellsto the basisof Estab— motilitydysfunction．Methods explorepathophysiologygastrointestinal lishedascites theascites andultrastructural model，identificatedcomponents，detectedpathologicalstudy pathol— and index．Results ofsmallintestinalinterstitial immunofluorescence ogy Cajal cells，c—kit apoptosis Tlympho— are inascites tumorassociated couldleadto and cytesmainlylymphocytescarcinoma，these lymphocytes damage ofintestinalinterstitialeens．ConclusionThetumorassociated intestinal apoptosis Cajal lymphocytesdamaged interstitial isthe basisof causedcan— cells，which Cajal pathophysiologygastrointestinalmotilitydysfunctionby cerousascites