大叶藻抑制松材线虫及携带细菌活性成分的分离和鉴定.pdf

Abstract Pinewilt destructivediseasefor causedthe disease(PWD)，a pine订ees，isby pine wood andadditionalbacteria．Inthis nematode(PWN)，Bursaphelenchus xylophilus andantibacterialofextractofZosteramarinawasstudied，and paper，nematicidal activity oneofactive isolatedand acid，was components，rosmarinic identified．Lightyellow followed wereobtainedfromextractsofz．marina solventextraction crystals through by onAB-8resinand lHNMRandHPLCwere chromatography crystallization．The to theisolated a rateofthe identify compound．Tohighyield crystal，single optimized get an results factor and were experimentperformed．The experimentsL9(34)orthogonal showedthatthe obtainedwasrosmarinicacid．The crystals optimal lightyellow extractionforrosmarinicacidwas：extractwith70％ethanolat40℃for3hours． process was ofRosAfromZ．marinawas Theextraction dosage1：50(WⅣ)．Thehighestyield 3．1 acidshowedeffectivenematicidal DW(driedweight)，Rosmarinic activity,of 3mg／g whichthe PWNat and72hwas1．18 24h，48h rag／g, LCso(50％lethalconcentration)to 1．05 andO．95 crudeextrac