气质联用法测定人血清游离脂肪酸李海静.pdfVIP

30 2 Vol.30　No.2 2009 3 Journal of Chinese Mass pectrometry ociety M ar.2009 李海静, 吴胜明, 方均建, 李　昱, 马昆鹏, 宋占军, 董方霆 (, 　100850) :-(GC-T OF-M )(FFA)。 , -/ / , , GC-T OF-M 。 , , , 33 min , / 20 C ～ C 。 FFA 12 24 , 、、、 , 。 :(FFAs);;-(GC-T OF-M ); :O 657.63　　:A 　　:1004-2997(2009)02-83-05 Determination of Free Fatty Acids in Human Serum by GC-TOF-MS LI Hai-jing, WU heng-ming, FANG Jun-jian, LI Yu, MA Kun-peng, ONG Zhan-jun, DONG Fang-ting (N a tiona l Cen ter of B iomed ica l A na ly s is, B eij ing 100850, Ch ina) Abstract:A modified method for determination of free fatty acids (FFAs)in human serum w as established by gas chromatography time-of flight mass spectrometry (GC-TOF-M ). Heptadecanoic acid methyl ester w as selected as internal standard.A fter derivatization by 2.5% H2 O4 /CH3 O H, the FFAs in serum w ere transformed into the fatty acid methyl es- ters, and N -hexane w as added to extract the products.The analysis of extract w as per- formed by GC-TOF-M .Better results w ere obtained with derivatization comparing w ith non-derivatization.At least 20 kinds of FFA s (C12 -C24 )in human serum are determinated by the modified method.The analysis is completed w ithin 33 min.The modified method show s potential useful for the scientific research to the disease correlating FFAs and lipido- mics due to the simplify, rapidity, high accuracy and enormous information. Key words:free fatty acids (FFAs);human serum ;gas chromatography time-of flight mass spectrometry (GC-TOF-M );derivatization 　　(free fatty acids, FFAs) 。 。 , FFA s [1] FFAs , 、 , FFAs 、、、 :2008-0