荧光假单胞菌gm5-1a鞭毛蛋白对黑松的作用机理及蛋白酶抑制剂防治松褐天牛的应用研究.pdf

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荧光假单胞菌gm5-1a鞭毛蛋白对黑松的作用机理及蛋白酶抑制剂防治松褐天牛的应用研究.pdf

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荧光假单胞菌gm5-1a鞭毛蛋白对黑松的作用机理及蛋白酶抑制剂防治松褐天牛的应用研究

Abstract Interactionbetween secreted GcM5—1Acarriedthe flagellin by尸．fluorescens by pine wood cellsofP nematode(PWN)(Bursaphelenchusxytophaus)andsuspension lethaleffectof to were thunbergii嬲well鹊the cells investigatedbyusing flagellinplant immunofluoresces meaSurementand aSsay,cellularstaining,conductivityelectrophoresis resultsshowedthatthereexistedadirectinteractionbetween analysis．The suspension cells and cellmembraneWaS was ofP．thunbergiiflagellin．The shrinkaged，thecytoplaSm nucleusWaSdividedinto micronuclearsandthe RNA concentrated，the many cytoplaSmic WaS inthetreatedcells．The ofthetreatedcellWas degraded tintingstrength strengthened withanincreaSeofculturemedia which allincrease accompanied conductivity，implied ofthe of cell of suspension analysisgenomic permeability membrane．Electrophoresis DNAconfirmedthatDNA inthetreated noevidentDNA breakagehappened cells，but ladderwasformed．We thatthelethalmodeofP cellscaused supposed thunbergii by isatypical flagellin apoptosis． In further and orderto the of constitutive verifytoxicityflagellin，a secretingexpressing 1 WaSconstructed．ThetiC ofPseudomonas plas