p16INK4a在早孕小鼠子宫内膜的表达及其对胚泡着床的影响.pdfVIP

Acta Physiologica Sinica , August 25, 2008, 60 4 : 547-552 547 Research Paper Expression of p16INK4a in mouse endometrium and its effect during blastocyst implantation * YANG Huan, XIE Yi, YANG Rong , WEI Sha-Li, XI Qiang Department of Reproductive Physiology, Chongqing Medical University, Chongqing 400016 Abstract: The expression of tumor suppressor gene p16INK4a in mouse endometrium during early pregnancy and its possible role in blastocyst implantation were investigated in the present study. Real-time fluorescent quantitative PCR FQ-PCR and immunohisto- chemistry were applied to detect p16INK4a mRNA and protein expressions in endometrium of un-pregnant and pregnant mice on day 2, 3, 4, 5, 7, respectively. In addition, p16INK4a antibody was injected into the horns of uteri in pregnant mice on day 3 and its effect during blastocyst implantation was detected in vivo. The higher expressions of p16INK4a mRNA and protein were observed in pregnant mice compared with that in un-pregnant mice, with a steady increase from day 2 to day 5 and reaching the maximal level on day 5 of pregnancy and then decreasing. p16INK4a antibody decreased the number of implanted blastocysts compared with that of saline-injected group. The results suggest that p16INK4a may be associated with apoptosis of luminal epithelial cells and decidual cells, coordinating decidualization of endometrium and invasion of trophoblastic cells. Thus, we presume that p16INK4a participates in the process of blastocyst implantation in mice. Key words: blastocyst implantation; endometrium; p16INK4a ; real-time fluorescent quantitative PCR; immunohistochemistry; in vivo p16INK4a 在早孕小鼠子宫内膜的表达及其对胚泡着床的影响 * 杨环，谢怡，杨戎 ，魏莎莉，郗强 重庆医科大学生殖生理教研室，重庆 400016 摘要：本研究旨在检测肿瘤抑制基因p16INK4a inhibitor of cyclin-dependent kinase 4a 在早孕小鼠子宫内膜中的表达规律，探讨 p16INK4a 在小鼠胚胎着床过程中的作用。采用荧光定量PCR FQ-PCR 和免疫组织化学方法分别检测未孕小鼠及孕小鼠第2 、3 、 4 、5 、7 天子宫内膜p16 INK4a mRNA 和蛋白的表达；子宫角注射p16 INK4a 抗体观察胚泡着床数。FQ-PCR 结果显示孕小鼠子 宫内膜组织p16 INK