《棉花耐盐碱性状的QTL定位》.pdfVIP

江苏农业学报(JiangsuJ.of Agr.Sci. ),2014,30(5):966~971 966 http:/ / 吴巧娟,刘剑光,赵摇 君,等. 棉花耐盐碱性状的QTL定位[J].江苏农业学报,2014,30(5):966鄄971. doi:10.3969/ j.issn.1000鄄4440.2014.05.007 棉花耐盐碱性状的QTL定位 吴巧娟,摇 刘剑光,摇 赵摇 君,摇 肖松华 (江苏省农业科学院经济作物研究所/ 农业部长江下游棉花与油菜重点实验室,江苏 南京 210014) 摇 摇 摘要:摇 以感盐碱棉花品系新洋718和耐盐碱棉花品系苏研 128构建的F 群体为材料,采用SSR分子标记构 2 建了包含47个位点和16个连锁群的棉花分子遗传图谱。 采用复合区间作图法对215个F 家系的相对盐害率进 2 颐 3 行QTL定位,检测到6 个与棉花耐盐碱性状相关的QTL,分别位于第 3、5、7、7、15 和 19 染色体上,分别解释 3郾23%、9郾86%、5郾77%、5郾28%、11郾31%和11郾24%的表型变异。 关键词:摇 棉花; 耐盐碱; SSR; QTL定位 中图分类号:摇 S562.032摇 摇 摇 文献标识码:摇 A摇 摇 摇 文章编号:摇 1000鄄4440(2014)05鄄0966鄄06 QTL mapping of tolerance to salt and alkali in cotton (Gossypium hirsutum L. ) WU Qiao鄄juan,摇 LIUJian鄄guang,摇 ZHAOJun,摇 XIAO Song鄄hua (Institute of Industrial Crops,JiangsuAcademy of Agricultural Sciences/ Key Laboratory of Cotton andRapeseed,Ministry of Agriculture,Nanjing 210014, China) 摇 摇 Abstract:摇 In this study,an F molecular mapping population derivedfrom the cross between salt alkali鄄sensitive 2 Xinyang718 and salt alkali tolerant Suyan 128wasdeveloped. A geneticlinkagemap comprisedof47 loci and 16 link鄄 age groups by SSR molecular marker was established. The composite interval mapping (CIM) methodwasusedto analyze the quantitative trait loci (QTLs) associatedwith salt injury rate in F families,and six QTLs of salt tolerance of cotton 2:3 were identified,which were located on chromosomes 3,5,7,7,15 and 19,respectively. The six QTLs could explain 2郾23%,9郾86%,5郾77%,5郾28%,11郾31% and 11郾24% of the