Purification and structural characterization of Chinese yam polysaccharide and its activities》.pdf
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Purification and structural characterization of Chinese yam polysaccharide and its activities》.pdf
Carbohydrate Polymers 117 (2015) 1021–1027
Contents lists available at ScienceDirect
Carbohydrate Polymers
j o u r na l h o me p a ge : w w w. elsev /locate/car bpol
Purification and structural characterization of Chinese yam
polysaccharide and its activities
Weifang Yang, Ying Wang, Xiuping Li, Ping Yu ∗
College of Food Science and Biotechnology, Zhejiang Gongshang University, 149Jiaogong Road, Hangzhou 310035, Zhejiang Province, People’s Republic of
China
a r t i c l e i n f o a b s t r a c t
Article history: Purification and structural characterization of Chinese yam polysaccharide were investigated and its
Received 14 July 2014 activities were analyzed. Results indicated that a single component polysaccharide with a molecular
Received in revised form weight of 16,619 Da was obtained after hot water extraction with sequential sevage deproteinization,
13 September 2014
HSCCC and Sephadex G-100 size-exclusion chromatography. The FTIR analysis showed that it had char-
Accepted 22 September 2014
Available online 22 October 2014 acteristic absorptive peaks and contained uronic acid. The methylation and GC–MS analysis showed
that it comprised of glucose and galactose with a molar ratio of 1.52:1, and that it mainly contained
1,3-linked-glc, 1-linked-gal and 1,6-linked-gal glycosidic bonds. 1H NMR and 13C NMR spectra analysis
Keywords:
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