Quaternary ammonium salt containing soybean oil An efficient nanosize gene delivery carrier for halophile green microalgal transformation》.pdf

Chemico-Biological Interactions 225 (2015) 80–89 Contents lists available at ScienceDirect Chemico-Biological Interactions journal homepage: www.elsevi /locate/chembioint Quaternary ammonium salt containing soybean oil: An efficient nanosize gene delivery carrier for halophile green microalgal transformation Fariba Akbari a,b,1, Ahmad Yari Khosroushahi c,d,⇑,1, Hamid Yeganeh e,⇑ a Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran b Student Research Committee, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran c Drug Applied Research Center, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran d Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran e Iran Polymer and Petrochemical Institute (IPPI), P.O. Box 14965/115, Tehran, Iran a r t i c l e i n f o a b s t r a c t Article history: Dunaliella salina, a halophile green microalga, is considered a robust photobioreactor and a remarkable Received 16 July 2014 cost beneficial system for the production of therapeutic recombinant proteins. In this study, with low Received in revised form 4 October 2014 overall cost, a proper cationic lipid was synthesized from renewable soybean oil as an efficient gene deliv- Accepted 7 October 2014 ery carrier for D. salina cells to create appropriate protein-producing transformed cell lines. To obtain an Available online 17 October 2014