Quenching of the electrochemiluminescence of RU-complex tagged shared-stem hairpin probes by graphene oxide and its application to quantitative turn-on detection of DNA》.pdf

Biosensors and Bioelectronics 70 (2015) 441–446 Contents lists available at ScienceDirect Biosensors and Bioelectronics journal homepage: /locate/bios Quenching of the electrochemiluminescence of RU-complex tagged shared-stem hairpin probes by graphene oxide and its application to quantitative turn-on detection of DNA Xiang Huang a, Xiaopeng Huang a, An Zhang a, Bangrong Zhuo a, Fushen Lu a, Yaowen Chen b, Wenhua Gao a,b,n a Department of Chemistry, Shantou University, Shantou, Guangdong 515063, PR China b Analysis Testing Center, Shantou University, Shantou, Guangdong 515063, PR China a r t i c l e i n f o a b s t r a c t Article history: Efficient and stable quenching of electrochemiluminescence (ECL) of tris(2,2’-bipyridine)-ruthenium(II) Received 22 January 2015 (Ru(bpy)32 þ )/tri-n-propylamine (TPrA) system by graphene oxide (GO) at the glassy carbon electrode Received in revised form (GCE) was reported. For figuring out the possible reasons of the quenching mechanism, the electro- 23 March 2015 chemical and ECL performance of GO, different reduction degree of reduced graphene oxide (RGOs) and Accepted 27 March 2015 Available online 28 March 2015 polymer wrapped GO modified GCEs were systematacially investigated. The results demonstrated that the oxygen-containing groups and poor electrical conductivity of GO, along with the distance between Keywords: GO and Ru(bpy)32 þ was suggeste