《Proteome analysis of hepatic non-parenchymal cells of immune liver fibrosis rats.》.pdf

SCIENCE CHINA LifeSciences · RESEARCH PAPER · March20l4 Vo1．57 No．3：303—314 doi：l0．1007／s11427—014-4619-．O Proteomeanalysisofhepaticnon-parenchymalcellsofimmune liverfibrosisrats ZHAoQianQian，FENGYanLing，JIAXiaoFang，Y]INLin，ZHENGYe。 0UYANG DongSheng ．ZHOUHongHao‘ ZHANG LiJun S‘hanghaiPublicHealthClinwalCenter,FudanUniversity．Shanghai201508．China； InstituteofClinicalPharmacology,PharmacogeneticsResearchInstitute，CentralSouthUniversity,Changsha410078，Chbm ReceivedMay20，2013；acceptedNovember20，2013；publishedonlineFebruary19，2014 Elucidationofthemechanismsofliverfibrogenesisisimportanttotreatliverfibrosis．Inthisstudy．weestablishedratmodels ofliverfibrosiswithstagesfromo_1，2，and3_4t04at2，4，6，and8weeks，respectively，bvinjectionofpigserum．Liverfi— brogenesiswasdetectedbyMasson’strichromestaining．Ratnon．-parenchymalcellsfNPCs)wereenriched4、foldbyPercoll densitygradientcentrifugation．Proteinextractsfrom NPCswerepreparedat4and8weeks，separatedbytwo--dimensional【 electrophoresis,andthenstainedwithCoomassieBlueG．250．At4weeks，wejdentified 18non．．redundantdifie~ntiallyex— pressedproteinsofwhichproteindisulfide一4someraseassociatedprotein3(PDIA3)andNDUVshowedconsistentexpression atproteinandmRNA levelsrfom 4to8weeks．PDIA3wasfoundtobedown．．regulatedbvWesternblottingintheratmodel andimmunohistochemicallyinhuman]liver．Ourresultsrevealedimportantaspectsofthepathogenesis／progressionofliverfi— brosisanddemonstratedimportantchangesinproteinexpressionlevdsofNPCqsatva