不同剂量黄芪对糖尿病大鼠脑干听觉诱发电位影响及其作用机制地研究.pdfVIP

Different doses of Astragalus on the intervention of the brainstem auditory evoked potential in diabetic rats Supervisor： Prof. LIU De-shan Postgraduate ：： AN Wen-can ABSTRACT Objective Observation to explore the interventional effect and mechanism of different doses of Astragalus on diabetic rat central nervous system functions. Methods We established diabetic rat model based on the theory that diabetes was Qi deficiency-oriented and oxidative stress was involved in diabetes central nervous system lesions. After treatment by different doses of Astragalus which functions to invigorate Qi, various indicators of electrophysiology, pathophysiology, biochemistry and molecular biology were detected. Results Astragalus can lower blood sugar, reduce the level of glycosylated hemoglobin, increase SOD levels, reduce the content of MDA and 8-OHdG, and inhibit the apoptosis of brain stem neurons, and significantly improve diabetic rat central nervous damage induced by hyperglycemia and oxidative stress. Conclusion Astragalus can significantly improve the central nervous system function in diabetic rats, to reduce the central nervous system damage, the mechanism may be achieved by lowering blood glucose and anti-oxidation Key word invigorating Qi; Astragalus; diabetes mellitus; rat; brainstem auditory evoked potentials; Oxidative stress; molecular biology; mechanism 目 录 引 言 1 材料与方法 3 一、材料 3 （一）实验动物 3 （二）主要仪器设备 3 （三）主要实验试剂 4 二、方法 4 （一）糖尿病大鼠模型复制方法及分组 4 （二）实验药物及给药方法 4 结 果 11 一、一般情况观察 11 （一）一般情况 11 （二）体重的变化 11 （三）血糖的测定 12 （四）BAEP 的检测 12 （五）血清糖化血红蛋白的测定 14