《Acidophilic bacterial community reflecting pollution level of sulphide mine impacted by acid mine drainage》.pdfVIP

J. Cent. South Univ. Technol. (2009) 16: 0223−0229 DOI: 10.1007/s11771−009−0038−y Acidophilic bacterial community reflecting pollution level of sulphide mine impacted by acid mine drainage WAN Min-xi(万民熙)1, YANG Yu(杨 宇)1, 2, QIU Guan-zhou(邱冠周)1, 2, 1 1 1 1, 2 XU Ai-ling(徐爱玲) , QIAN Lin(钱 林) , HUANG Zhi-ying(黄芝英) , XIA Jin-lan(夏金兰) (1. School of Resources Processing and Bioengineering, Central South University, Changsha 410083, China; 2. Key Laboratory of Biometallurgy of Ministry of Education, Central South University, Changsha 410083, China) Abstract: To reveal the impact of mining on bacterial ecology around mining area, bacterial community and geochemical characteristics about Dabaoshan Mine (Guangdong Province, China) were studied. By amplified ribosomal DNA restriction analysis and phylogenetic analysis, it is found that mining pollution greatly impacts the bacterial ecology and makes the habitat type of polluted environments close to acid mine drainage (AMD) ecology. The polluted environment is acidified so greatly that neutrophil and alkaliphilic microbes are massively dead and decomposed. It provided organic matters that can make Acidiphilium sp. rapidly grow and become the most bacterial species in this niche. Furthermore, Acidithiobacillus ferrooxidans and Leptospirillum sp. are also present in this niche. The amount of Leptospirillum sp. is far more than that of Acidithiobacillus ferrooxidans, which indicates that the concentration of toxic ions is very high. The conclusions of biogeochemical analysis and microbiological monitor are identical. Moreover, because the growth of Acidithiobacillus ferrooxidans and Lep