《不同品种羽衣甘蓝生长期色素含量变化规律研究 Studies on Change of Pigment Contents During Different Developmental Stages Among Several Flowering Kale Cultivars》.pdfVIP

中国农学通报 2013,29(10):144-149 Chinese Agricultural Science Bulletin ‘黑珍珠’番茄植株再生体系的研究 1 1 1 1 1 1 2 1 许飞云 ，张赵男 ，侯雨辰 ，王英正 ，王 龙 ，王 珂 ，徐 进 ，赵 宇 1 2 （晋中学院，山西榆次 030600；中国科学院遗传与发育生物学研究所农业资源研究中心，石家庄 050021） 摘 要：为了研究‘黑珍珠’番茄植株再生，以‘黑珍珠’番茄幼嫩叶片为外植体诱导愈伤组织，通过愈伤组 织诱导培养、愈伤组织分化培养、不定芽增殖培养、生根培养和试管苗移栽，建立高效快速的‘黑珍珠’番 茄再生体系。结果表明：最适宜的诱导叶片愈伤组织的培养基为MS+ 1.0 mg/L 6-BA+ 0.1 mg/L NAA， 叶片外植体愈伤组织诱导率最高可达98.2% ；诱导出的愈伤组织在MS+ 1.5 mg/L 6-BA+ 0.2 mg/L IBA 培养基上能很好的分化出不定芽；MS+4.0 mg/L KT+ 0.01 mg/L IBA 培养基可实现不定芽芽增殖；最适 宜的生根培养基为1/2MS+ (0.05~0.08) mg/L NAA ，试管苗移栽成活率达92%。 关键词：‘黑珍珠’番茄；愈伤组织；移栽 中图分类号：S641.2 文献标志码：A 论文编号：2012-3176 The Study of Efficient Plant Regeneration of Solanum lycopersicum ‘Black ’ XuFeiyun1,ZhangZhaonan,HouYuchen,WangYingzheng,WangLong,WangKe,XuJin,ZhaoYu1 1 1 1 1 2 1 1 (Jinzhong University,YuciShanxi030600; 2Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences,Shijiazhuang050021) Abstract: Procedures for callus induction, shoot regeneration, multiplication of shoot and rooting from regenerated shoots of ‘Black’tomato were optimized by the study of several factor. The highest callus inductionfrequencywasobtainedfromleafexplantsonMSmediumcontaining 1.0 mg/Lbenzylaminopurine (6-BA)with 0.1 mg/L α-naphthaleneaceticacid(NAA).Theformedcalluswastransferredtoregeneration mediumsupplementedwith 6-BAandIBAandthebesthormonaltreatmenttoshootregenerationwasthe mediumwith 1.5 mg/L 6-BAand 0.2 mg/L 3-indolebutyric(IBA).Themediumcontaining4.0 mg/Lkinetin (KT)and0.01 mg/LIBAwasfoundtobeth