《中国部分地方黄牛品种线粒体D-loop区的遗传变异与进化分析 Genetic Variation of Mitochondrial D-loop Region and Evolution Analysis in Some Chinese Cattle Breeds》.pdfVIP

Journal of Genetics and Genomics (Formerly Acta Genetica Sinica) June 2007, 34(6): 510-518 Research Article Genetic Variation of Mitochondrial D-loop Region and Evolution Analysis in Some Chinese Cattle Breeds 1 1, 2,① 3,① 3 1 4 Shangang Jia , Hong Chen , Guixiang Zhang , Zhigang Wang , Chuzhao Lei , Ru Yao , 3 Xu Han 1. College of Animal Science and Technology, Northwest AF University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China; 2. Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou 221116, China; 3. National Center for Preservation and Utilization of Genetic Resources of Domestic Animals and Forages, National Animal Husbandry Veteri- nary Service, Beijing 100094, China; 4. Department of Psychology, Educational Science College, Shandong Normal University, Jinan 250014, China Abstract: The complete mitochondrial D-loop region from 123 individuals in 12 Chinese cattle breeds and two individuals in Ger- many Yellow cattle breed was sequenced and analyzed. The results were shown as follows: 93 variations and 57 haplotypes were detected, and the average number of nucleotide difference was 22.708, nucleotide diversity (π) was 0.0251 ± 0.00479, and haplo- type diversity (Hd) was 0.888 ± 0.026, indicating very high genetic diversity in Chinese cattle breeds. In the Neighbor-Joining tree, 13 cattle breeds were divided into two main clades, Bos taurus and Bos indicus ; new Clade Ⅲ had only one sequence from Apei- jiaza cattle breed in Tibet, which was similar to that of yak at a higher level tha