《副溶血性弧菌的亚致死热损伤和复苏条件分析 Sublethal Thermal Injury and Resuscitation Conditions of Vibrio parahaemolyticus》.pdfVIP

227 ※生物工程 食品科学 2013,Voi．34,No．21 副溶血性弧菌的亚致死热损伤和复苏条件分析 陈婵娟，宁喜斌水，丛健，盛骥春 上海海洋大学食品学院，上海水产品加工及贮藏工程技术研究中心，上海 201306 摘要：目的：研究不同温度条件下副溶血性弧菌的亚致死热损伤程度，最适宜的复苏条件以及复苏后细菌的生 长特性。方法：运用传统的固体平板法对不同热处理条件下副溶血性弧菌VpBJl．1997细胞的亚致死损伤率进行检 测，然后通过液体法绘制细菌复苏生长曲线，并结合不同复苏时间下通过扫描电镜观察副溶血性弧菌的形态特征和 复苏后亚致死损伤率的测定，最终确定准确的亚致死损伤和复苏条件。结果：副溶血性弧菌在45C条件下处理时间 10min，亚致死损伤率达到最高；35C复苏3h后，损伤程度最低。结论：不同温度下的亚致死损伤率说明，副溶血 性弧菌在30℃条件下培养可以降低亚致死损伤导致的漏检率。 关键词：副溶血性弧菌；亚致死损伤；复苏 andResuscitationConditionsofVibrio SublethalThermal parahaemolyticus Injury CHEN Xi．bin木，CONGJian，SHENGJi-chun Chan-juan，NING ResearchCenter and ofFoodScienceand ShanghaiEngineering ofAquatic-productProcessingPreservation，College Technology, Ocean ShanghaiUniversity,Shanghai201306，China the ofsublethalthermalinHbrio resuscitation parahaemolyticus，the Abstract：Objective：Tostudydegree injury optimum ofthe strainsandthe characteristicsofresuscitative sublethallyinjured growth strains．Methods：Firstly， temperature wedetectedthe ofsublethalinVibrio BJl．1997atdifferent degress injury parahaemolyticus temperaturesbyusing curves Hbrio BJI．1997 traditionalsolid of methods．Then，resuscitativegrowth sublethallyinjuredparahaemolyticus agar wereestablished cultivationmethod．The characteristicsofthe and byusingliquid morphological sublethallyinjuries andthe ofsublethalandresuscitation theresuscitativestrainswereobserved SEM conditions byusing optimal injury of BJI．1997weredetermined．Results：TheextentofsublethalinHbrio temperatureHbrioparahaemolyticus highest injury was after10mintreatmentat wellasthelowest instrainsresuscitated BJl．1997obtained 45℃as parahaemolyticus injury at35℃for3 tothemeasurementofsublethalatdifferent of h．Conclusion：According injury temperatures．theprobability Hbrio rateat30℃Canbereduced． parahaemolyticusfalse-negative Keywords：Vibrioparahaemolyticus；sublethalinjury；resuscitation 中图分类号：R155 文献标志码：A doi：10．7506／spkxl002-6630·座机电话号码6 致死损伤菌在非选择性培养基中可以形成菌落，而在选 副溶血性弧菌 Vibrioparahaemolyticus，Vp 是革兰 氏阴性嗜盐细菌，隶属弧菌科中的弧菌属，是一种人兽 择性培养基上不能