《Fibulin-5在盆腔器官脱垂患者子宫骶韧带中的表达》.pdfVIP

·论著· 王睿 杨欣 【摘要】 目的 研究Fibulin一5在子宫骶韧带组织中的表达及与盆腔器官脱垂程度之间的关系。方法 应用实时定量RT—PCR和Western Fibulin一5 (Po．05)。结论Fibulin一5表达的降低可能促进了盆腔器官脱垂的发生。 【关键词】 Fibulin一5；盆腔器官脱垂；弹性蛋白；实时定量PCR；免疫蛋白印迹法 of inuterosacralof The Fibulin一5 expression ligamentpelvicorganprolapsedpatients W，ANGR札i。yANGXi竹． Obstetricsand First 100034，China) (Departmentof Gynecology，PekingUniversityHospital，Be毛iing To the ofFibulin一5inuterosacralof with [Abstract] expression patients Objectiveinvestigate ligament wereobtained from46 duringhysterectomy pelvicorganprolapsed(POP)．MethodsSpecimens prospectively womenwith and28eomrol levelsof andmRNAoffibulin一5in pelvicorganprolapse patients．Expressionprotein chain uterosaeral weremeasuredWesternblot andreal—time reac— ligaments by analysis quantitativepolymerase Fibulin一5 weredetectableinboth tOcontrol mR— tions．Results expression groups．Comparedgroup，Fibulin一5 NA was lessthanthatinPOP VS of expressionsignificantly group(O．33+0．22 inP()Puterosacral tissues(O．59士0．31)thanthatin Fibulion一5 ligaments proteinsignificantly(PO．05)lower levels were withthe normaluterosacral tissues(10．09-t--0．46，P=O．02)．AndofFibulin一5decreased ligaments