《HPLC-DAD同时测定山茱萸饮片中5个成分的含量》.pdfVIP

HPLC-DAD同时测定山茱萸饮片中5个成分的含量 杜伟锋，蔡皓，蔡宝昌’(江苏省中药炮制重点实验室．南京中医药大学，南京210029) 食子酸、5．羟甲基糠醛(5．HMF)、莫诺苷、獐牙菜苷、马钱素。方法应用Zorbax C18柱，以0．1％磷酸溶液和O．1％磷酸 乙腈溶液为流动相，梯度洗脱，流速1 浓度的线性关系良好(rO．999 评价和控制山茱萸饮片质量提供依据。 关键词：高效液相二极管阵列检测法；山茱萸；没食子酸；5-羟甲基糠醛；莫诺苷；獐牙菜苷；马钱素 中图分类号：B917 文献标识码：A 文章编号：1001—2494(2008)17—1342—05 SimultaneousDeterminationofFiveActiveConstituentsinComus HPLC-DAD officinalisby DU Medicines Chinese Hao，CAI ofChinese Universityof Wei—feng，CAI Bao-chang’(Jiangsu研LaboratoryProcessing，№，讲，Ig 210029，China) Medicine，Nanking Toestablisha methodwithdiode ABSTRACT：OBJECTIVE highperformanceliquidchromatographic array simultaneousdeterminationoffiveactivecomtituentsin fourmain of DAD)‘Dr Comus面钰i嬲协fromplace Five withaZorbaxcolumn elution acidwater- constituents雠他analyzed simultaneously C18 bygradient using0．1％phosphoric acidacetonitrilesolution鹧themobile columnWBJ5maintainedat30℃．TheflowrateWag1mL· solution-0．I％phosphoric phase．The rain一1．Thedetection wasSetat and284ImLRESULTSAUtIIeconstituentsshowed 218，240 iinearity(r wavelength good 0．999 the ofthetested recoveriesofthemethodwerebetween98．16％and102．53％． 6)inrange concentration，the average CONCLUSIONThevalidatedmethodhasthe of and itsintrinsiccontrol advant