《Optimization for Microbial Degradation of Dibenzothiophene by Pseudomonas sp. LKY-5 Using Response Surface Methodology》.pdfVIP

China Petroleum Processing and Petrochemical Technology Scientific Research 2014, Vol. 16, No. 1, pp 19-26 March 30, 2014 Optimization for Microbial Degradation of Dibenzothiophene by Pseudomonas sp. LKY-5 Using Response Surface Methodology Li Lin; Zhao Chaocheng; Liu Qiyou; Zhang Yunbo; Liu Chunshuang; Xue Jianliang (College of Chemical Engineering, China University of Petroleum, Qingdao 266580 ) Abstract: In this research, the degradation of dibenzothiophene (DBT) was investigated by using Pseudomonas sp. LKY-5 isolated from oil contaminated soil. The response surface methodology (RSM) based on the Box-Behnken design (BBD) was applied for evaluating the interactive effects of four independent variables including substrate concentration, tempera- ture, pH and agitation rate on the DBT removal response. A total of 29 experiments for four factors at three levels were conducted in present study. A second-order regression model was then developed, and the analysis of variance (ANOVA) illustrated that the proposed quadratic model could be utilized to navigate the design space. The value of determination coeffi- cient (R2=0.953 4) indicated a satisfactory agreement between the quadratic model and the experimental data. It was found that DBT removal was more significantly affected (P0.000 1) by substrate concentration compared with other three parameters. An 100% degradation of DBT could be obtained by Pseudomonas sp. LKY-5 at a substrate concentration of 100 mg/L. Key words: degradation; dibenzothiophene; Pseudomonas sp.; response surface methodology; Box–Behnken design 1　Introduction treatment methods to remove these pollutants before be-