《Survivin siRNA协同5-FU抑制MCF-7细胞的增殖》.pdfVIP

2006;26(3) 南方医科大学学报(J South Med Univ) 251 $ $ - Synergism between a siRNA targeted to survivin and 5 FU in inhibiting MCF-7 cell proliferation in vitro 1 2 1 1 1 ZHANG Shu-gun , ZHANG Shu-hui , XUE Xin-huan , WANG Xi-j ing , JIANG Jian-tao 1 2 Department of Oncology, Second Hospital, Xian Jiaotong University, Xian 7 10004, China; Department of Chemical Engineering, Northwestern Polytechnical University, Xian 710072, China Abstract: Objective To investigate the role of small interfering RNA (siRNA) targeted to survivin in combination with 5-fluorouracil (5-FU) in inhibiting the proliferation of MCF-7 cells. Methods A siRNA targeted to survivin was synthesized and transfected into MCF-7 cells via lipofectin. Changes of the cell growth activity in response to combined treatment with survivin siRNA and 5-FU or 5-FU treatment alone was evaluated by MTT assay. The ! method of Jin Zhenjun was used to evaluated synergism between the synthesized siRNA and 5-FU. Results Treatment with 5 nmol/L siRNA reduced the IC of 50 5-FU from 4.42 to 1.18 !g/ml, and the inhibitory effect of c