VesnaSvetlii,NadicaIvoevi,SolvegKovaandVerautiCenterforMarineandEnvironmentalResearch,RuerBokoviInstitute,POBox180,10002….docVIP

REVISED CHARGE DISPLACEMENT BY ADHESION AND SPREADING OF A CELL Vesna Svetli?i?, Nadica Ivo?evi?, Solveg Kova? and Vera ?uti? Center for Marine and Environmental Research, Ru?er Bo?kovi? Institute, POBox 180, 10 002 Zagreb, Croatia Keywords: electrical adhesion signals, dropping mercury electrode as adhesion sensor, Dunaliella tertiolecta as a model cell Abstract The potentiostatic control of surface charge density and interfacial tension of an electrode immersed in an aqueous electrolyte solution offers a possibility for direct studies of non-specific interactions in cell adhesion. Unicellular marine alga, Dunaliella tertiolecta (Chlorophyceae) of micrometer size and flexible cell envelope was used as a model cell and 0.1 M NaCl as supporting electrolyte. The dropping mercury electrode acted as in situ adhesion sensor and the electrochemical technique of chronoamperometry allowed measurement of the spread cell-electrode interface area and the distance of the closest approach of a cell. The adhesion and spreading of a single cell at the mercury electrode causes a displacement of counter-ions from the electrical double layer over a broad range of the positive and negative surface charge densities (from +16.0 to –8.2 (C/cm2). The flow of compensating current reflects the dynamics of adhesive contact formation and subsequent spreading of a cell. The adhesion and spreading rates are enhanced by the hydrodynamic regime of electrode’s growing fluid interface. The distance of the closest approach of an adherent cell is smaller or equal to the distance of the outer Helmholz plane within the electrical double layer, i.e. 0.3-0.5 nm. There is a clear evidence of cell rupture for the potentials of maximum attraction as the area of the contact interface exceeded up to 100 times the cross-section area of a free cell. Introduction Many of essential features of cell deformation due to adhesion can be considered in the context of an abstract representation of cells as complex flui