《青霉菌灭活菌丝体诱导烟草抑制消减杂交cDNA文库的构建-论文》.pdfVIP

26 中国烟草科学 Chinese Tobacco Science 2014-04 ，35 （2 ） cDNA  1 1# 1 1 1 1 1* 1* 2 1. 650091 2. 650106 DMPDMP SSHcDNA SSH 356 102 77 Blastn 47 Blastx 26 7 2 40.3%23.4% 14.9% 8.5%4.3%4.3%4.3% cDNA SSH S435.72 1007-5119201402-0026-06 DOI 10.13496/j.issn.1007-5119.2014.02.005 Establishment of a Suppression Subtractive Hybridization cDNA Library of Tobacco Induced by Dry Mycelium of Penicillium Chrysogenum 1 1# 1 1 1 1 CHEN Zhuangzhuang , XIE Hong , ZHONG Yu , ZHANG Pengyuan , LONG Chunrui , CHEN Lin , WANG 1* 1* 2 Jianguang , CHEN Suiyun , LI Xiujun (1. School of Life Sciences, Yunnan University, Kunming 650091, Yunnan, China; 2. Baoteng Biochemical Technology Co.,Ltd., Kunming 650106, Yunnan, China) Abstract: Dry mycelium of Penicillium chrysogenum (DMP) could protect plant from disease. But its mechanism of induction and signaling pathways was unclear. Analyzing the genes expression in the plant treated by DMP was a good way to find out how inducing agent works. SSH technology was used to establish a cDNA library of tobacco induced by Dry mycelium of Penicillium chrysogenum. In the library 356 positive clones were obtained, of which 102 clones were selected for sequencing and 77 were sequenced successful. The result of Blastn showed that 47 genes’ functions were obtained in the library. The result of Blastx indicated that 26 genes had homologous sequences and 7 genes showed high similarities with putative