Hematopoesis.pptVIP

Parvovirus Classification Classification of Viruses Prior to 1966 an array of different names/classifications Moscow 1966 – International Congress of Microbiology International Committee on Taxonomy of Viruses (ICTV) A single taxonomic scheme for all viruses Naming and classification 70 Study Groups who provide the information Regular reports (currently 8th) ICTV classification ‘a virus species is a polythetic class of viruses that constitute a replicating lineage and occupy a particular ecological niche’ (7th report) Polythetic class - several properties in common, but not necessarily a single common defining property Hierarchy of recognised viral taxa: (Order); Family; (Subfamily); Genus; Species ‘Other groupings (from clade to super-family), may communicate useful descriptive information in some circumstances but they have no formally recognized taxonomic meaning’ (8th report, p4) Demarcation criteria: When is a new virus a new virus? Pairwise sequence identity profiles: well resolved peaks between strains, species and genera. Different virus families show similar overall patterns. Genetic analysis should provide validation of taxonomic assignments. Parvoviridae Non-enveloped 22-24nm icosahedral virion 4 - 5.6 kb ss linear DNA genome ITRs at 5’ and 3’ ends Encode no polymerase Dependant on cellular/other viral proteins for DNA replication 2 large ORF NS (or Rep) proteins VP (or Cap) proteins Parvovirus Classification Host range -2 subfamilies Densovirinae – insects Parvovirinae – vertebrates Autonomous/dependant replication + , –, or +/- strand packaging Identical or different 5’ and 3’ ITRs 1, 2 or 3 promoters Sequence phylogeny Parvovirus Taxonomy Erythrovirus Genus Encapsidate equivalent numbers of + and – strand upon extraction anneal to form ds DNA Long palindromic ITR (~383 nt) ‘Identical’ ITR at 5’ and 3’ ends Single mRNA promoter Polyadenylation signals mid genome and 3’ end Efficient replication in erythroid precursors