Studyontheanti-depressiveeffectofreboxetineinvolvedinnon-transporterinhibition.docVIP

瑞波西汀抗抑郁作用的非转运体抑制机制研究 胡小娅，李娜，费慧芝，王涵，文威，周岐新（重庆医科大学药理学教研室，重庆 400016） [摘要]目的 探讨瑞波西汀抗大鼠抑郁作用与改善氧化应激平衡、HPA轴功能及BDNF表达关系。方法 60只♂SD大鼠，随机分为对照组（NG）、模型组（MG）、灌胃给予瑞波西汀（0.7mg/（kg*d））正常组(RNG)和灌胃给予瑞波西汀（0.7mg/（kg*d））模型组（RMG）。采用孤养结合慢性轻度不可预见刺激（CUMS）方法建立大鼠抑郁模型。以高架迷宫法、生物化学方法、放射免疫法免疫组化染色RT-PCR评价大鼠抑郁行为，检测皮质丙二醛（MDA）含量、超氧化物岐化酶（SOD）和过氧化氢酶（CAT）的活性，血清CORT海马BDNF海马BDNF下丘脑CRF mRNA表达进入开臂的次数和时间以及在开臂向下探究的次数 anti-depressive effect of reboxetine involved in non-transporter inhibition HU Xiaoya，LI Na，FEI Huizhi，WANG Han,WEN Wei,ZHOU Qixin (Dept of Pharmacology, Chongqing Medical Univercity, Chongqing 400016, China) Abstract] Aim To investigate the relation between the anti-depression effect of reboxetine and the oxidative/anti-oxidative stress balance as well as HPA axis function and expression of BDNF in depressive model rats induced by chronic unpredictable mild stress（CUMS）. Methods sixty male SD rats were randomly divided into normal group（NG），model group（MG），reboxetine()-treated normal group（RNG），and reboxetine( )-treated model group（RMG.CUMS with solitary condition was taken to establish rat depression model. The elevated plus maze（EPM），biochemistry methods524331288@； 周岐新（1947-），男，教授，博士生导师，研究方向：神经药理学，通讯作者，Tel：023E-mail：cqzhouqx@ radioimmunity，immunohistochemistry，and reverse transcription polymerase chain reaction (RT-PCR) were used to determine the depression behaviors of rats，MDA level and SOD and CAT activities in brain cortex.，serum corticosterone (CORT) level，BDNF protein，and mRNA expressions of BDNF in hippocampus and CRF in hypothalamus，respectively. Results It was shown that the MDA level, CORT concentration, and CRF mRNA expression significantly increased with obviously decreasing behavior parameters，SOD and CAT activities，and expressions of BDNF mRNA and protein in MG，compared with NG. The treatment of roboxetine obviously blunted the changes above caused by CUMS. However treatment of roboxetine had no influences on NG rats. Conclusion The treatment of reboxetin