ResearchonrelationshipbetweenleptinreceptorgeneGIn223Argpolymorphismandtype2diabetesmellitus.docVIP

瘦素受体基因Gln223Arg多态性与2型糖尿病关系的研究( 张阳丹1 李革1 张名均2 滕小春3 陈春全3 唐晓君1*（400016 重庆，重庆医科大学：公共卫生学院1；400050 重庆，重庆市九龙坡区第一人民医院2；400081 重庆，重钢总医院3） 【摘要】 目的： 研究瘦素受体基因Gln223Arg多态性与2型糖尿病的关系。 方法： 采用病例对照研究方法，随机抽取重庆市2所医院的糖尿病门诊和住院初诊患者及其他疾病和门诊健康体检者共336人，其中病例组（糖尿病患者）172人，对照组（非糖尿病者）164人，调查其糖尿病危险因素，同时用聚合酶链反应--限制性片段长度多态性(PCR-RFLP)方法检测其瘦素受体Gln223Arg基因多态性，分析其与2型糖尿病的关系。 结果： 该人群AA、AG、GG的基因型分布频率为0.015，0.307和0.678，A和G等位基因频率分别为0.202和0.798，基因型频率分布符合H-W平衡（x2= 3.081，P =0.079x2=6.96 P=0.008）P=0.05、0.03、.0001、0.03Gln223Arg突变与2型糖尿病发病的有关。 结论：瘦素受体基因Gln223Arg多态性与2型糖尿病可能有关。 【关键词】 瘦素受体基因 Gln223Arg 2型糖尿病 【中图法分类号】 R181 【文献标识码】A Research on relationship between leptin receptor gene GIn223Arg polymorphism and type2 diabetes mellitus ZHANG Yangdan, TANG Xiaojun, LI GE, ZHANG Mingjun, et al. (1Epidemilolgical Department of Health Public school, ChongQing Medical University, Chongqing 400016; 2 the Jiulongpo first peoples hospital, Chongqing, 400050; 3 Chonggang general hospital,Chongqing, 400081)． [Abstract]：Objectiveo study the possible relationship between the polymorphism of leptin receptor (LEPR) gene Gln223Arg and type 2 diabetes. Methods A case-control study was adopted to investigate 236 patients of two hospitals in Chongqing (the Jiulongpo first peoples hospital and the Chonggang general hospital), including patients on the first visit to diabetes outpatient and inpatient departments, patients with other diseases, and patients coming for the physical examination, of whom 44 people were excluded because of variable defects, a total of 336 patients were then assigned into two groups, 172 patients in the case group (with diabetes) and 164 in the control group (without diabetes). Then we investigate their risk factors of diabetes, and meanwhile test the polymorphism of leptin receptor (LEPR) gene Gln223Arg by the polymerase chain reaction—restriction fragment length polymorphism (PCR - RFLP), to analyze its relationship with type 2 diabetes. Results The genotype frequency distribution