一种新型输尿管支架管的可溶解包被薄膜及生物相容性研究.docVIP

一种新型输尿管支架管的可溶解包被薄膜及生物相容性研究 郑宗钊 王瑨 宋波 李传贵 张银甫 黄赤兵(400037，重庆，第三军医大学新桥医院泌尿外科) [摘要] 目的 为一种带纳米铁牵引线输尿管支架管筛选出一种安全的可溶解包被材料并检测该材料及纳米铁线的生物相容性。方法 通过对三种材料所形成薄膜溶解特性的比较选出所需材料。进一步研究所选材料溶液浓度及薄膜厚度与溶解时间的关系。在SD大鼠背部进行该材料覆盖的纳米铁牵引线的肌肉植入试验，检测其生物相容性。结果 初步确定用聚乙烯醇制备可溶解薄膜。聚乙烯醇薄膜溶解时间与溶液浓度无关（P＞0.05），与薄膜厚度呈正相关（P＜0.05），可利用薄膜厚度调节牵引线释放时间。生物相容性：术后1-2周，光镜下见所埋线周围有大量炎性细胞浸润；术后4周炎性细胞数量明显减少；术后12周牵引线周围仅见少量炎性细胞。结论 聚乙烯醇能作为可溶解包被薄膜材料用于该新型输尿管支架管。聚乙烯醇及其覆盖的纳米铁牵引线具有良好的生物相容性。 [关键词] 输尿管支架管；聚乙烯醇；生物相容性 Experimental study of a dissolvable film coated on a novel ureteral stent and their biocompatibility Zheng Zongzhao, Wang Jin, Song Bo, Li Chuangui , Huang Chibing(Department of Urology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China) [Abstract] Objective To find a safe and dissolvable material which can be used as a dissolvable coated film in a novel ureteral stent with nanoscale iron traction and evaluate the biocompatibility of this material and the nanoscale iron traction. Methods Determine the tentative material according to the comparison of three films made of three different materials. Do further study to analyze the relation between the material’s solution concentration and the film’s dissolving time or the film’s thickness. Test the material and the nanoscale iron traction’s biocompatibility by the muscle implant test in SD rats. Results Through the comparative research, polyvinyl alcohol was chose as the tentative material. The polyvinyl alcohol film’s dissolving time was related to higher levels of the film’s thickness(P0.05), and was not associated with solution concentration(P0.05). Biocompatibility: One and two weeks after the implant surgery, there were abundant capillaries and inflammatory cells around the implanted traction. After four weeks, inflammatory cells were significant reduced, but there were still abundant capillaries and some fibroblasts could be seen around the capillaries. At the 12th week there were only little inflammatory cells, the quantity of capillar