质谱和联用新技术在烟草化学中的应用研究.pdf

摘要 断裂，产生对甲氧基苄基自由基。首次通过中间体的研究，明确两种糖苷不同的 裂解机理，为糖苷加香应用提供了理论基础。 4、UPLC-MS3 方法对卷烟烟气中TSNAs 分析研究 研究了超高压液相色谱串联质谱联用技术（Ultra-high-pressure liquid chromatography tandem mass spectrometry, UPLC-MS/MS ）建立的UPLC-MS3 新方 法对卷烟烟气中四种亚硝胺进行快速、高选择性、高灵敏度测定。通过粒径小于 2μm 的UPLC 柱的使用及对色谱条件的优化，实现了短时间内四种烟草特有亚 硝胺的色谱基线分离。根据不同烟草特有亚硝胺的母离子、二级母离子在质谱中 进行二次碎裂产生的三级离子，选择出各自适合定量的三级子离子，建立了适合 复杂烟气基质中亚硝胺分析的高选择性MS3 方法，提高了复杂基质中测定的准 确性。 5、UPLC-QqLIT 对卷烟烟气中TSNAs 分析研究 研究了超高压液相色谱四级杆（Quadrupole, Q ）线性离子阱(Linear ion trap, LIT)串联质谱联用技术建立快速、高灵敏度、可以同时定性定量的UPLC-MS/MS 新方法测定四种烟草特有亚硝胺。离子阱质谱在全扫描模式下具有较高的灵敏度 和离子阱对离子具有聚集的作用，利用QqLIT 串联建立的MS/MS 新方法在全扫 Enhanced product ion, EPI 描模式下具有高的灵敏度。通过增强子离子扫描模式（ ） 下，提取不同的离子作为定量离子可以有效避免传统的HPLC-MS/MS 方法在测 定低含量烟草特有亚硝胺时存在的干扰问题。 关键词：光致电离质谱 裂解气相色谱质谱 高效液相色谱串联质谱 三级离子扫 描糖苷潜香物质烟草特有亚硝胺卷烟烟气 II Abstract Abstract Smoking and health is an important scientific problem which is concerned with the development of society. In recent years, many scientists of tobacco chemistry have been committed to minimizing the toxicity of harmful ingredients on human body and the amount of cigarette tar which contained primary harmful ingredients. However, as the cigarette tar decreased, a lot of flavor components would be lost which caused the cigarette smell falling at the same time. In order to effectively implement and evaluate the goal of harm reduction, on the one hand flavor additives need to be studied to compensate the loss of flavor while the cigarette tar is reduced; on the other hand some new analytical methods for fast, selective and sensitive determination of some strongly carcinogenic ingredients in cigarette smoke need to be established. I