Conservation of Iris bismarckiana Regel (Iridaceae) Using Plant Regeneration via Somatic Embryogenesis.pdfVIP
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Conservation of Iris bismarckiana Regel (Iridaceae) Using Plant Regeneration via Somatic Embryogenesis.pdf
JournalofAgriculturalScienceandTechnologyA4(2014)605-611
Earliertitle:JournalofAgriculturalScienceandTechnology,ISSN 1939—1250
… N6
Conservationof/r/sbismarckianaRegel(Iridaceae)
UsingPlantRegenerationviaSomaticEmbr、,,og|,eneSIS
SawsanA.Oran andAhmadH.AI—Gabbiesh2
1.DepartmentofBiologicalSciences,FaculytofSciences,UniversityofJordan.Amman11942,Jordan
2.DepartmentofAlliedMedicalSciences,ZarqaCollege,A1-BalqaAppliedUniversity,Salt1911LJordan
Received:April29,2014/Published:July20,2014
Abstract:Inanattempttopropagateandconservetherare,showybulbousplantsoflrisbismarckiana,newlyrecordedtotheflora
ofJordanandtocontributetotheconservationthewildIrisspeciesinJordan,asimplerapid,timeconsumingprotocolhasbeen
developedusingplantregenerationviasomaticembryogenesis.Somaticembryogenesiswasinducedinzygoticembryocultureon
MurashigeandSkoog(MS)mediumsupplementedwith2,4一dichlorophenoxyaceticacid(8mg/L)asthesoleplantgrowthregulator,
wherebothembryogenesiscalliandsomaticembryoswereinduced.Separationofembryosfrom embryoclusterswasnecessary to
enhancetherfequencyofgermination.Germinationwasstimulatedbyseparationofembryossuccessfullyrfom embryoclustersand
transferred onto flesh MS medium .Data obtained wereanalyzed asacompleterandom design with three replications.Calli
rfagmentsthatweretransferredtoembryogenesisinductionmedium (EIM)producedwhiteembryo—likeglobularstructureswithin
twoweeks.Withinthreemoreweeks,clustersofsturcturesatvariousstagesofdevelopmentcouldbefoundonthesamecallus.The
appliedtechniqueisrewardingandencouragingforfurtherresearchontheendangeredwildspeciesofIrisinJordan.
Keywords:Conservation,somaticembryogenesis,plantregeneration,flowerbase,MS,callusinductionmedium (CIM),lris
bismarckiana.
1.IntroductiOn bulbsorspliRingofrhizomes(rhizomatousIris).In
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