下调转录因子HOXB7抑制胰腺癌BXPC3细胞侵袭及迁移机制研究.pdf

郭 贞，等 下调转录因子 HOXB7抑制胰腺癌BXPC3细胞侵袭及迁移机制研究 下调转录因子 HOXB7抑制胰腺癌 BXPC3细胞侵袭及迁移机制研究 郭 贞，江卫华，金子 良，焦 锋，王理伟 SilencingoftranscriptionfactorHOXB7 inhibited invasionandmigrationofpancreatic cancercelllineBXPC3 GuoZhen，JiangWeihua，JinZiliang，JiaoFeng，WangLiwei DepartmentofOneology，ShanghaiJiaotongUniversityAffiliatedShanghaiFirstPeopleHospital，Shanghai201620，China． 【Abstract】 Objective：PurposeTorisethesequence—specificshRNAtointerferencetheexpressionoftranscrip— tionfactorHOXB7andinvestigateitseffectoncellinvasionand migrationinpancreaticcancercellllneBXPC3． Methods：ExpressionofHOXB7mRNA andprotein innormalhumna pancreaticductalepithelialcellsHPDE and pancreaticcancercelllinesBXPC3weredetectedbyReal—timePCR andWesternblot．ExpressionofHOXB7mRNA andproteininHOXB7shRNA groupandcontrolshRNA groupwasdetectedbyReal—timePCR andWesternblot． TheinvasionandmigrationabilitywereobservedbyTranswellinvasionassayandscratchassay．Results：Real—time PCR andWestern blotanalysisshowedHOXB7mRNA andproteinexpressioninpna creaticcancercelllineBXPC3 wassignificantlyhigherthannomr alpancreaticductalepithelial cellsHPDE．TheexpressionofHOXB7mRNA and proteinwereinhibitedinBXPC3ceilstransfectedHOXB7shRNA．CellinvasionandmirgationalsoreducedinBXPC3 cellstransfectedHOXB7shRNA．Conclusion：H0XB7shRNA caneffectivelyinhibitHOXB7expression．alsoreduce theabilityofinvasionandmigrationinBXPC3cells． 【Keywords】pancreaticcancer；BXPC3；HOXB7；invasion；mirgation Modern Oncology2015，23(10)：1344—1348 【摘要】 目的：研究在胰腺癌细胞株BXPC3中转染shRNA干扰转录因子HOXB7的表达后，BXPC3细胞侵袭 及迁移能力的变化。方法：Real—timePCR及Westernblot印迹方法分别检测HOXB7基因mRNA及蛋白在正 常人胰腺导管上皮细胞HPDE、胰腺癌细胞株 BXPC3中的表达；在胰腺癌细胞株BXPC3细胞中转染 HOXB7 shRNA及阴性对照shRNA，mRNA及蛋白水平检测转录因子HOXB7的干扰效果；Transwell侵袭实验检测各 组细胞的侵袭能力 ；划痕实验检测各组细胞迁移能力的变化。结果：Real—timePCR及Westernblot结果显示 HOXB7基因mRNA及蛋白在BXPC3细胞