基于激发态能量转移机理比率型荧光探针的研究进展有机化学.pdfVIP

DOI: 10.6023/cjoc201409036 综述与进展 有机化学 Chinese Journal of Organic Chemistry REVIEW 基于激发态能量转移机理比率型荧光探针的研究进展 陈忠林a 李红玲a 韦 驾a 肖 义b 于海波*,a (a 辽宁大学环境学院 沈阳 110036) (b 大连理工大学 精细化工国家重点实验室 大连 116024) 摘要 激发态能量转移(Excitation Energy Transfer, EET)作为一类重要的光物理现象, 被广泛用于比率型荧光探针和分 子灯标的设计以及 DNA 检测等多个领域. 影响 EET 效率的两个重要因素是供受体间的空间距离和光谱交盖, 通过调 节供受体间的空间距离或光谱重叠程度来调控能量转移过程, 实现对目标客体的双波长比率检测. 综述了基于不同供 受体荧光团的 EET 体系、供受体间的连接方式对能量转移效率的影响, 以及通过调控供受体间光谱重叠程度或空间距 离, 获得识别不同客体的比率型荧光探针, 并对 EET 机理的比率型荧光探针的设计以及未来在生物成像和医学检测等 领域的应用进行了展望. 关键词 荧光探针; 激发态能量转移; Förster 能量转移; 比率型荧光探针; 荧光发色团 Recent Progress in Ratiometric Fluorescent Probes Based on Excitation Energy Transfer Mechanism Chen, Zhonglina Li, Honglinga Wei, Jiaa Xiao, Yib Yu, Haibo*,a a ( College of Environmental Sciences, Liaoning University, Shenyang 110036 ) b ( State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024) Abstract Excitation energy transfer (EET) is one of the vital photophysical phenomenons, which is widely used in many applications, such as the design of ratiometric fluroesent probes, molecular beacon, DNA analysis, and so on. The process of energy transfer from donor to acceptor can be regulated by two factors: the spatial distance between donor and acceptor, and the spectral overlaps between donor emission and acceptor absorption, which results that